Fiber - FISH mapping

Figure 1

Human Y chromosome in interphase. "FIBER-FISH" with Y-specific DNA probes RBM (red) and TSPY (green)

Literatur: Conrad C, Hierl T, Gläser B, Taylor K, Zeitler S, Chandley AC, Schempp W (1996) High-resolution fluorescence in situ hybridization of RBM- and TSPY-related cosmids on released Y chromatin in humans and pygmy chimpanzees. Chromosome Res 4: 201-206 ( > Abstract )

Figure 2

High-resolution FISH of DAZG5 (green) from the 5' end and DAZG21 (red) from the 3' end of the DAZ gene on an extended Y chromatin structure from the S-phase. Seven longer DAZ signal stretches (genes/pseudogenes) can clearly be defined by the differentially labelled probes of the 5' and 3' end of the DAZ gene. The bar corresponds to I0 micro m.

Literatur: Gläser B, Yen P, Schempp W (1998) Fibre-fluorescence in situ hybridization unravels apparently seven DAZ genes or pseudogenes clustered within a Y-chromosome region frequently deleted in azoospermic males. Chromosome Res 6:481-486 ( > Publication in full text )

Figure 3

Examples of FISH mapping on DAPI-stained prometaphase human Y chromosomes. (a) Three-color FISH of DAZ, SMCY and CDY. Note that SMCY (yellow) co-hybridises with the proximal CDY (blue) cluster in Yq11.22. (b) Two-color FISH of TSPY and XKRY. Note that a faint TSPY (pink) signal is located within the XKRY (yellow) signal in Yq11.22. Centromeres are marked by small bars.

Figure 4

Fiber-FISH contig of human NRY. The contig starts in proximal Yp (upper left) and ends with the long arm heterochromatin (lower right). Color code for the signal arrays of the genes is given at the upper right. Signal arrays for the Ycen, XKRY and Yqh markers for orientating the fibers are shown in yellow. By the numbered star signals each fiber-FISH structure can be identified and inspected in the foregoing original figures in more detail. Bar corresponds to 20 μm.

Literature: Röttger S, Yen PH, Schempp W (2002) A fiber-FISH contig spanning the non-recombining region of the human Y chromosome. Chromosome Res 10: 621-635 ( > Publication in full text )