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S. maltophilia Multilocus Sequence Typing Database Sequence Typing Database

Catalogue of services offered by the Hygiene and Molecular Laboratory

• Genotyping... more
• Identification of antibiotic resistance genes ... more
• Bacterial identification ... more
• Resistance testing against antibiotics, among other things natural substances ... more
• Reference stock... more
• Methods applied ... more

Genotyping

Staphylococcus aureus (PFGE, spa, MLST/SBT), Enterococcus faecium (PFGE, MLVA, MLST/SBT),
Escherichia coli (AFLP), Klebsiella spp. (AFLP), Enterobacter spp. (AFLP), Serratia marcescens (AFLP),
Pseudomonas aeruginosa (AFLP, MLVA, MLST/STB), Stenotrophomonas maltophilia (AFLP, MLST/STB), Acinetobacter spp. (AFLP), Legionella pneumophila.(PFEG, AFLP, MLST/SBT)

(PFGE: Pulsfield gelelectrophoresis spa: Staphylococcal Protein A Typing; AFLP: Amplified fragment length polymorphism; MLVA: Multiple locus variant number of tandem repeat analysis, MLST: Multi locus sequence typing; SBT: Sequence based typing)

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Identification of antibiotic resistence genes

• Sequence-based identification of ß-lactamase-coded sequences: Extended spectrum beta-lactamase (Groups TEM, SHV, CTX-M, OXA), Metallo-beta-lactamases (Groups IMP, VIM, GIM, SPM, SIM), carbapenemases (groups NMC, IRS, IMI, KPC, GES) and AmpC.
• Gram-positive resistance genes: mecA, SCCmec, vanA, vanB, MLS-group (ermA, ermC, msrA)
• Quinolone resistance genes (gyrA, parC, qnr)

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Bacterial identification

• Biochemical (API, VITEK)
• Molecular biological (16S rRNA, cpn60, gyrB, mip for Legionella spp.)
• Virulence factors: S. aureus: Panton-Valentin leucocidin; E. coli: Aerobactin iutA und Yersiniabactin fyuA, capsule synthesis genlocus kpsMT II, invasive factor ibeA, serum resistance factor traT, adhesion factors papA und papG, iron-regulated gene homologue adhesin iha; Determination of phylogenetic groups.

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Antimicrobial susceptibility testing against antibiotics, among other things natural substances

• Micro- and macrodilution-broth methods (NCCLS/CLSI)
• Agar dilution (NCCLS/CLSI), E-Test (on request)
• Bactericidal testing (NCCLS/CLSI)
• Synergism testing (chekerboard method)

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Reference stock

Provision of characterised (genotyped) reference stock (Staphylococcus aureus, Enterococcus faecium, Escherichia coli, Klebsiella spp., Enterobacter spp., Serratia marcescens, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Acinetobacter spp., Legionella spp.)

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Methods applied

Typing methods are available for the following pathogens:
Staphylococcus aureus, Enterococcus faecium, Escherichia coli, Klebsiella spp., Enterobacter spp., Serratia marcescens, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Acinetobacter spp., Legionella pneumophila.

Typing methods include:
Macrorestriction analysis (PFGE), Amplified fragment length polymorphism (AFLP), random amplification of polymorphic DNA (RAPD), multiple locus variant numbers of tandem repeat analysis (MLVA), spa typing, multi locus sequence typing (MLST).

Identification of antibiotic resistance genes
Sequence-based identification of beta-Laktamase-coding genes::

• Extended spectrum beta-lactamases (groups TEM, SHV, CTX-M, OXA)
• Metallo-beta-lactamases (groups IMP, VIM, GIM, SPM, SIM)
• Carbapenemases (groups NMC, IRS, IMI, KPC, GES)
• AmpC beta-lactamases (groups MOX, CMY, LAT-4, BIL-1, DHA, ACC, MIR-1T, ACT-1, FOX)
• Quinolone resistence genes (gyrA, parC, qnr)
• Gram-positive resistance genes: mecA, SCCmec, vanA, vanB, MLS-groups (ermA, ermC, msrA)

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