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Arbeitsgruppe Prof. Dr. med. M. Werner / PD Dr. S. Laßmann

Unsere Arbeitsgruppe „Molekulare Tumorpathologie“ beschäftigt sich mit soliden Tumoren und  hämatologischen Neoplasien sowie deren Vorstufen.

Die enge interdisziplinäre Vernetzung mit Arbeitsgruppen des Tumorzentrums Ludwig-Heilmeyer (CCCF) und der Albert-Ludwigs-Universität sowie mit anderen Kliniken und Instituten im In- und Ausland stärkt unser Team.

Themen:

  • Molekulare Mechanismen der Karzinogenese
  • Genetische Instabilität und Signaltransduktion
  • Molekulare Marker für Therapie-Prädiktion und Prognose

       

Spezielle Labortechniken (in vitro, in situ):

Zellen und Gewebe: siRNA, FACS, Laser-Mikrodissektion, Gewebearrays, 2D-, 3D-Mikroskopie

DNA: Fluoreszenz-In-Situ Hybridisierung (FISH), Quantitative-PCR, Genchips

mRNA: RNA- In Situ Hybridisierung (RISH), Quantitative RT-PCR, Genchips

Proteine: Western Blots, Immunhistochemie, Immunfluoreszenz 

Das Team:

Martin Werner   Prof. Dr. med.  0761 270-80060 
Silke Laßmann  PD Dr.   0761 270-80620 
Konrad Aumann  Dr. med. 0761 270-81260
Ilona Kohler  Dr. med.   0761 270-80210 
Annette May   Dr. med. 0761 270-81280
Thorsten Wiech   Dr. med. 0761 270-80240
Claudia Münch  Dr. rer. nat.   0761 270-80310
      
Fahima Sijare  cand. Dr. rer. nat.   0761 270-80310
Julian Benckendorf  cand. Dr. med.     
Christiane Fichter   cand. Dr., Molekul. Medizin    0761 270-80310
Lucas Spohn  cand. Dr. med.      
      
Theresa Ahrens  Diplomantin, Molekul. Medizin 0761 270-80310
Jasmine Roth  MTA 0761 270-80810
Anja Schöpflin  MTA  0761 270-80470
Katja Thurig  MTA 0761 270-80470 

 

Kooperierende Institutionen:

Tumorzentrum Ludwig-Heilmeyer; Comprehensive Cancer Center Freiburg

HelmholtzZentrum München; Institut für Pathologie

Memorial Sloan Kettering Cancer Center, Dept. of Pathology, New York, USA

Huazhong University of Science and Technology; Tongji Medical University, Union Hospital, Dept. of Obstetrics and Gynecology

Laufende Förderung:

Deutsche Forschungsgemeinschaft
2010 - 2013 SFB850, Teilprojekt C5: „Die Rolle von Rezeptortyrosinkinasen-Signalwegen bei der Invasion und Metastasierung von Ösophaguskarzinomen“ http://www.sfb850.uni-freiburg.de

Deutsche Jose Carreras Leukämie Stiftung 
2009 - 2011: „Relevanz der ID-Proteine als Zielstrukturen für neue therapeutische Ansätze bei akuten Leukämien“

Mushett Family Foundation
2004 - 2010: „Programm grant in cancer research“

Bundesministerium für Bildung und Forschung
2008 - 2012: “Integriertes Behandlungszentrum Chronische Immundefizienz”

Bundesministerium für Bildung und Forschung / Oncotest GmbH
2010 -: „Evaluierung prädiktiver Gensignaturen von Bevazizumab für Patienten mit kolorektalen Karzinomen“

Deutsche Krebshilfe
2008 - 2010: „Onkologisches Spitzenzentrum, Förderung Tumorbank“

Robert-Bosch Stiftung
2008 - 2010: „Deutsch-Chinesische Forschungsanbahnung: Identifizierung von Biomarkern in Ovarialkarzinomen“

Publikationen

• Link zu Pubmed 

• Aktuelle Publikationen (2010-2004):

2010

Schlachter M, Reisert M, Herz C, Schluermann F, Lassmann S, Werner M, Burkhardt H, Ronneberger O. Harmonic Filters for 3 D Multi-Channel Data: Rotation Invariant Detection of Mitoses in Colorectal Cancer. IEEE Trans Med Imaging. 2010 Jun 7. [Epub ahead of print]

Kayser G, Kassem A, Sienel W, Schulte-Uentrop L, Mattern D, Aumann, K, Stickeler E, Werner M, Passlick B, zur Hausen A. Lactate-dehydrogenase 5 is overexpressed in non-small cell lung cancer and correlates with the expression of the transketolase-like protein 1. Diagn Pathol. 2010 Apr 12;5:22

Neumann J, Feuerhake F, Kayser G, Wiech T, Aumann K, Passlick B, Fisch P, Werner M, zur Hausen A. Gene expression profiles of lung adenocarcinoma linked to histopathological grading and survival but not to EGF-R status: a microarray study. BMC Cancer. 2010 Mar 2;10:77

Manthey C, Mern DS, Gutmann A, Zielinski AJ, Herz C, Lassmann S, Hasskarl J. Elevated endogenous expression of the dominant negative basic helix-loop-helix protein ID1 correlates with significant centrosome abnormalities in human tumor cells. BMC Cell Biol. 2010 Jan 14;11:2

2009

Stumpf M, Hasenburg A, Riener MO, Jütting U, Wang C, Shen Y, Orlowska-Volk M, Fisch P, Wang Z, Gitsch G, Werner M, Lassmann S. Intraepithelial CD8-positive T lymphocytes predict survival for patients with serous stage III ovarian carcinomas: relevance of clonal selection of T lymphocytes. Br J Cancer. 2009 Nov 3;101(9):1513-21.

Lassmann S, Danciu M, Müller M, Weis R, Makowiec F, Schulte-Mönting J, Hopt UT, Werner M. Aurora A is differentially expressed and regulated in chromosomal and microsatellite instable sporadic colorectal cancers. Mod Pathol. 2009 Oct;22(10):1385-97.

Wiech T, Stein S, Lachenmaier V, Schmitt E, Schwarz-Finsterle J, Wiech E, Hildenbrand G, Werner M, Hausmann M. Spatial allelic imbalance of BCL2 genes and chromosome 18 territories in nonneoplastic and neoplastic cervical squamous epithelium. Eur Biophys J. 2009 Jul;38(6):793-806.

Markert S, Lassmann S, Gabriel B, Klar M, Werner M, Gitsch G, Kratz F, Hasenburg A. Alpha-folate receptor expression in epithelial ovarian carcinoma and non-neoplastic ovarian tissue. Anticancer Res. 2008 Nov-Dec;28(6A):3567-72.

Harder J, Engelstaedter V, Usadel H, Lassmann S, Werner M, Baier P, Otto F, Varbanova M, Schaeffner E, Olschewski M, Blum HE, Opitz OG. CpG-island methylation of the ER promoter in colorectal cancer: analysis of micrometastases in lymph nodes from UICC stage I and II patients. Br J Cancer. 2009 Jan 27;100(2):360-5.

Lassmann S, Kreutz C, Schoepflin A, Hopt U, Timmer J, Werner M. A novel approach for reliable microarray analysis of microdissected tumor cells from formalin-fixed and paraffin-embedded colorectal cancer resection specimens. J Mol Med. 2009 Feb;87(2):211-24.

2008

Lassmann S, Kreutz C, Schoepflin A, Hopt U, Timmer J, Werner M. A novel approach for reliable microarray analysis of microdissected tumor cells from formalin-fixed and paraffin-embedded colorectal cancer resection specimens. J Mol Med. 2008 Dec 6. [Epub ahead of print]

Wellner U, Mattern D, Keck T. Clinical Challenges and Images in GI. Gastroenterology. 2008 Nov 12

Wiech T, Nikolopoulos E, Hausmann M, Walch A, Werner M, Fisch P.A Case of Heterogeneous Breast Cancer with Clonally Expanded T-Cells in the HER2+ and Metastasis of the HER2- Tumor Cells. Breast J. 2008 Sep-Oct;14(5):487-91.

Bauer VL, Braselmann H, Henke M, Mattern D, Walch A, Unger K, Baudis M, Lassmann S, Huber R, Wienberg J, Werner M, Zitzelsberger HF. Chromosomal changes characterize head and neck cancer with poor prognosis. J Mol Med. 2008 Sep 23.

Brücher BL, Keller G, Werner M, Müller U, Lassmann S, Cabras AD, Fend F, Busch R, Stein H, Allescher HD, Molls M, Siewert JR, Höfler H, Specht K. Using Q-RT-PCR to measure cyclin D1, TS, TP, DPD, and Her-2/neu as predictors for response, survival, and recurrence in patients with esophageal squamous cell carcinoma following radiochemotherapy. Int J Colorectal Dis. 2009 Jan;24(1):69-77

Wiech T, Nikolopoulos E, Weis R, Langer R, Bartholomé K, Timmer J, Walch AK, Höfler H, Werner M. Genome-wide analysis of genetic alterations in Barrett's adenocarcinoma using single nucleotide polymorphism arrays. Lab Invest. 2008 Jul 28. [Epub ahead of print]

Riener MO, Nikolopoulos E, Herr A, Wild PJ, Hausmann M, Wiech T, Orlowska-Volk M, Lassmann S, Walch A, Werner M. Microarray comparative genomic hybridization analysis of tubular breast carcinoma shows recurrent loss of the CDH13 locus on 16q. Hum Pathol. 2008 Jul 23. [Epub ahead of print] 

Kassem A, Schöpflin A, Diaz C, Weyers W, Stickeler E, Werner M, Zur Hausen A.Frequent detection of Merkel cell polyomavirus in human Merkel cell carcinomas and identification of a unique deletion in the VP1 gene. Cancer Res. 2008 Jul 1;68(13):5009-13. 

Wiech T, Nikolopoulos E, Lassman S, Heidt T, Schöpflin A, Sarbia M, Werner M, Shimizu Y, Sakka E, Ooka T, zur Hausen A. Cyclin D1 expression is induced by viral BARF1 and is overexpressed in EBV-associated gastric cancer. Virchows Arch. 2008 Jun;452(6):621-7.

Lassmann S, Opitz OG. The new look of colorectal cancer stem cells. Gastroenterology. 2008 Apr;134(4):1262-4.

Arndt S, Wiech T, Mader I, Aschendorff A, Maier W. Rare extracranial localization of primary intracranial neoplasm. Diagn Pathol. 2008 Apr 16;3:14.

Wittel UA, Wiech T, Chakraborty S, Boss B, Lauch R, Batra SK, Hopt UT. Taurocholate-induced pancreatitis: a model of severe necrotizing pancreatitis in mice. Pancreas. 2008 Mar;36(2):e9-21.

2007

Lassmann S, Shen Y, Jütting U, Whiele P, Walch A, Gitsch G, Hasenburg A, Werner M. [Aurora-a is a predictive marker for stage III epithelial ovarian cancers] Verh Dtsch Ges Pathol. 2007;91:225-32.

Lassmann S, Tang L, Capanu M, Brabletz T, Schöpflin A, Zur Hausen A, Gonen M, Kemeny N, Shia J, Klimstra D, Werner M. Predictive molecular markers for colorectal cancer patients with resected liver metastasis and adjuvant chemotherapy. Gastroenterology. 2007 Dec;133(6):1831-9

Arndt S, Wiech T, Mader I, Maier W. Entire infrasellar craniopharyngioma simulating clival chordoma. Otolaryngol Head Neck Surg. 2007 Dec;137(6):981-3.

Neumann HP, Vortmeyer A, Schmidt D, Werner M, Erlic Z, Cascon A, Bausch B, Januszewicz A, Eng C. Evidence of MEN-2 in the original description of classic pheochromocytoma. N Engl J Med. 2007 Sep 27;357(13):1311-5

Göbel H, Gloy J, Neumann J, Wiech T, Pisarski P, Böhm J. Donor-derived small cell lung carcinoma in a transplanted kidney. Transplantation. 2007 Sep 27;84(6):800-2.

Wiese AH, Auer J, Lassmann S, Nährig J, Rosenberg R, Höfler H, Rüger R, Werner M. Identification of gene signatures for invasive colorectal tumor cells. Cancer Detect Prev. 2007;31(4):282-95

Rauser S, Weis R, Braselmann H, Feith M, Stein HJ, Langer R, Hutzler P, Hausmann M, Lassmann S, Siewert JR, Höfler H, Werner M, Walch A. Significance of HER2 low-level copy gain in Barrett's cancer: implications for fluorescence in situ hybridization testing in tissues. Clin Cancer Res. 2007 Sep 1;13(17):5115-23

Rischke HC, Momm F, Henke M, Wiech T, Frommhold H. Does radiation prevent 5-fluorouracil-induced colitis in the early phase of radiochemotherapy? A case report and literature review. Strahlenther Onkol. 2007 Aug;183(8):459-63.

Thomas-Kaskel AK, Mattern D, Köhler G, Finke J, Behringer D. Reticulated platelet counts correlate with treatment response in patients with idiopathic thrombocytopenic purpura and help identify the complex causes of thrombocytopenia in patients after allogeneic hematopoietic stem cell transplantation. Cytometry B Clin Cytom. 2007 Jul;72(4):241-8. 

Lassmann S, Shen Y, Jütting U, Wiehle P, Walch A, Gitsch G, Hasenburg A, Werner M. Predictive value of Aurora-A/STK15 expression for late stage epithelial ovarian cancer patients treated by adjuvant chemotherapy. Clin Cancer Res. 2007 Jul 15;13(14):4083-91

Lassmann S, Schuster I, Walch A, Göbel H, Jütting U, Makowiec F, Hopt U, Werner M. STAT3 mRNA and protein expression in colorectal cancer: effects on STAT3-inducible targets linked to cell survival and proliferation. J Clin Pathol. 2007 Feb;60(2):173-9

Lassmann S, Weis R, Makowiec F, Roth J, Danciu M, Hopt U, Werner M. Array CGH identifies distinct DNA copy number profiles of oncogenes and tumor suppressor genes in chromosomal- and microsatellite-unstable sporadic colorectal carcinomas. J Mol Med. 2007 Mar;85(3):293-304

2006

Henke M, Mattern D, Pepe M, Bézay C, Weissenberger C, Werner M, Pajonk F. Abstract Do erythropoietin receptors on cancer cells explain unexpected clinical findings? J Clin Oncol. 2006 Oct 10;24(29):4708-13

Reiter R, Gais P, Jütting U, Steuer-Vogt MK, Pickhard A, Bink K, Rauser S, Lassmann S, Höfler H, Werner M, Walch A. Aurora kinase A messenger RNA overexpression is correlated with tumor progression and shortened survival in head and neck squamous cell carcinoma.

Clin Cancer Res. 2006 Sep 1;12(17):5136-41

Lassmann S, Werner M. A commentary on the article "Prevalence of the mismatch repair-deficient phenotype in colonic adenomas arising in HNPCC patients--results of a 5-year follow-up study". Int J Colorectal Dis. 2006 Oct;21(7):642-4

Bähr A, Schulte-Vallentin M, Hasse J, Werner M, Jüttner E, Uhl M, Niemeyer C, Kontny U. [Extrathoracal pulmonary sequestration in the differential diagnosis of paravertebral tumors in the neonate] Klin Padiatr. 2006 Jan-Feb;218(1):13-5

Gerlach U, Kayser G, Walch A, Hopt U, Schulte-Mönting J, Werner M, Lassmann S. Centrosome-, chromosomal-passenger- and cell-cycle-associated mRNAs are differentially regulated in the development of sporadic colorectal cancer. J Pathol. 2006 Mar;208(4):462-72

Lassmann S, Hennig M, Rosenberg R, Nährig J, Schreglmann J, Krause F, Poignee-Heger M, Nekarda H, Höfler H, Werner M. Thymidine phosphorylase, dihydropyrimidine dehydrogenase and thymidylate synthase mRNA expression in primary colorectal tumors-correlation to tumor histopathology and clinical follow-up. Int J Colorectal Dis. 2006 Apr;21(3):238-47

2005

Gabriel B, Watermann D, Hancke K, Gitsch G, Werner M, Tempfer C, Hausen A. Increased expression of matrix metalloproteinase 2 in uterosacral ligaments is associated with pelvic organ prolapse. Int Urogynecol J Pelvic Floor Dysfunct. 2006 Sep;17(5):478-82

Kontny U, Boppana S, Jung A, Goebel H, Strahm B, Peters A, Dormann S, Werner M, Bader P, Fisch P, Niemeyer C. Post-transplantation lymphoproliferative disorder of recipient origin in a boy with acute T-cell leukemia with detection of B-cell clonality 3 months before stem cell transplantation. Haematologica. 2005 Nov;90 Suppl:ECR27.

Lassmann S, Gerlach UV, Technau-Ihling K, Werner M, Fisch P. Application of BIOMED-2 primers in fixed and decalcified bone marrow biopsies: analysis of immunoglobulin H receptor rearrangements in B-cell non-Hodgkin's lymphomas. J Mol Diagn. 2005 Nov;7(5):582-91

Friebe V, Keck T, Mattern D, Schmitt-Graeff A, Werner M, Mikami Y, Adam U, Hopt UT. Serous cystadenocarcinoma of the pancreas: management of a rare entity. Pancreas. 2005 Aug;31(2):182-7

Wiech T, Walch A, Werner M. Histopathological classification of nonneoplastic and neoplastic gastrointestinal submucosal lesions. Endoscopy. 2005 Jul;37(7):630-4

Kayser G, Gerlach U, Walch A, Nitschke R, Haxelmans S, Kayser K, Hopt U, Werner M, Lassmann S. Numerical and structural centrosome aberrations are an early and stable event in the adenoma-carcinoma sequence of colorectal carcinomas. Virchows Arch. 2005 Jul;447(1):61-5

Gabriel B, Denschlag D, Göbel H, Fittkow C, Werner M, Gitsch G, Watermann D. Uterosacral ligament in postmenopausal women with or without pelvic organ prolapse. Int Urogynecol J Pelvic Floor Dysfunct. 2005 Nov-Dec;16(6):475-9

Wiech T, Timme S, Riede F, Stein S, Schuricke M, Cremer C, Werner M, Hausmann M, Walch A. Human archival tissues provide a valuable source for the analysis of spatial genome organization. Histochem Cell Biol. 2005 Mar;123(3):229-38

Bredel C, Lassmann S, Pollack IF, Knoth R, Hamilton RL, Volk B, Werner M, Bredel M. DNA topoisomerase IIalpha and Her-2/neu gene dosages in pediatric malignant gliomas. Int J Oncol. 2005 May;26(5):1187-92

2004

Walch A, Specht K, Braselmann H, Stein H, Siewert JR, Hopt U, Höfler H, Werner M. Coamplification and coexpression of GRB7 and ERBB2 is found in high grade intraepithelial neoplasia and in invasive Barrett's carcinoma. Int J Cancer. 2004 Dec 10;112(5):747-53

Hausmann M, Cremer C, Linares-Cruz G, Nebe TC, Peters K, Plesch A, Tham J, Vetter M, Werner M. Standardisation of FISH-procedures: summary of the Second Discussion Workshop. Cell Oncol. 2004;26(3):119-24

von Dobschuetz E, Walch A, Werner M, Hopt UT, Adam U. Giant ancient schwannoma of pancreatic head treated by extended pancreatoduodenectomy. Pancreatology. 2004;4(6):505-8

Albrecht B, Hausmann M, Zitzelsberger H, Stein H, Siewert JR, Hopt U, Langer R, Höfler H, Werner M, Walch A. Array-based comparative genomic hybridization for the detection of DNA sequence copy number changes in Barrett's adenocarcinoma. J Pathol. 2004 Jul;203(3):780-8

Neeff H, Obermaier R, Technau-Ihling K, Werner M, Kurtz C, Imdahl A, Hopt UT. Solitary fibrous tumour of the liver: case report and review of the literature. Langenbecks Arch Surg. 2004 Aug;389(4):293-8

Lassmann S, Bauer M, Rosenberg R, Nekarda H, Soong R, Rüger R, Höfler H, Werner M. Identification of occult tumor cells in node negative lymph nodes of colorectal cancer patients by cytokeratin 20 gene and protein expression. Int J Colorectal Dis. 2004 Mar;19(2):87-94.

Kawakami N, Lassmann S, Li Z, Odoardi F, Ritter T, Ziemssen T, Klinkert WE, Ellwart JW, Bradl M, Krivacic K, Lassmann H, Ransohoff RM, Volk HD, Wekerle H, Linington C, Flügel A. The activation status of neuroantigen-specific T cells in the target organ determines the clinical outcome of autoimmune encephalomyelitis. J Exp Med. 2004 Jan 19;199(2):185-97.

2003 und vorher:

Link zu Pubmed 

(p)

 

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