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Arbeitsgruppe Prof. Dr. P. Fisch
Laboratory Fisch
Links: Lab Main page (this one)
Projects (under devepolment)
Group members (under devepolment)
One major focus of our work is the human innate immune system. We are particularly interested in the functional role of gammadelta T cells, Natural Killer (NK) cells and NKT cells in tumor defence. We study the role of NK cells following hematopoietic stem cell transplantation in the graft-versus-leukemia response. We are also interested in the development of human natural killer cells from immature precursors in the bone marrow, NK cells in the thymus and in the peripheral lymphoid organs. In addition, we are interested in human gammadelta T cells with regard to the molecular composition of the gammadelta TCR, its functional role in antigen recognition and human diseases.
We have expertise in the cloning, surface marker and functional analyses of human T- and NK cell clones. In addition, we have established tools for monitoring the effector cells of the human immune system by analysing the repertoire of alphabeta T cells, gammadelta T cells and B cells by spectratyping of the variable region genes, as well as by flow cytometry. We also study the repertoire of NK cells for the expressed inhibitory and activating NK receptors by up to 8-color flow cytometry and by Q-PCR. We use molecular cloning to derive gene expression constructs, various techniques to transfer genes (such as HLA class I and others) into cell lines and other target cells in order to study the effects on the interaction with lymphocytes. We use Northern and Southern blots, PCR, RT-PCR, Q-PCR and Affymetrix microarray technologies for analysis of gene expression.
Another interest of our lab is molecular pathology, such as Laser microdissection to isolate cells of interest, followed by molecular analysis for clonality of lymphocytes, microsatellite stability or gene expression.
Paul Fisch, Group leader and Professor of Molecular Pathology
1959 Born in Bratislava, Czechoslovakia
1979-1985 Studies of Medicine in Köln, Germany, Minneapolis, MN and Madison, WI, USA
1986-1987 Resident in Neurology, Köln, and the Medical Policlinic, Würzburg, Germany
1987-1990 Fellow of the Leukemia Society of America in the laboratory of Dr. Paul Sondel, Madison, WI USA
1991-1992 Fellow of the DFG, Laboratory of Molecular Biology, Cambridge, England in the group of Terry Rabbitts
1993-1996 Special Fellow of the Leukemia Society of America, Department of Medicine (Hematology and Oncology), University of Freiburg, Germany
1996 Habilitation for Immunology, Faculty of Medicine, University of Freiburg, Germany
1997-1998 DFG Heisenberg Fellowship, University of Tübingen, Department of Immunology (Prof. Dr. H.-G- Rammensee), Group leader
1998 Habilitation for Immunology and Molecular Genetics, Faculty of Medicine, University of Tübingen, Germany
Since 1998 Professor of Molecular Pathology (C3), Department of Pathology, University of Freiburg Hospital
Ten most important publications
1. Fisch, P., Malkovsky, M., Braakman, E., Sturm, E., Bolhuis, R.L., Prieve, A., Sosman, J.A., Lam, V.A., and Sondel, P.M. 1990. / T cell clones and natural killer cell clones mediate distinct patterns of non-major histocompatibility complex-restricted cytolysis. J Exp Med 171:1567-1579.
2. Fisch, P., Malkovsky, M., Kovats, S., Sturm, E., Braakman, E., Klein, B.S., Voss, S.D., Morrissey, L.W., DeMars, R., Welch, W.J., et al. 1990. Recognition by human V9/V2 T cells of a GroEL homolog on Daudi Burkitt's lymphoma cells. Science 250:1269-1273.
3. Fisch, P., Boehm, T., Lavenir, I., Larson, T., Arno, J., Forster, A., and Rabbitts, T.H. 1992. T-cell acute lymphoblastic lymphoma induced in transgenic mice by the RBTN1 and RBTN2 LIM-domain genes. Oncogene 7:2389-2397.
4. Fisch, P., Forster, A., Sherrington, P.D., Dyer, M.J., and Rabbitts, T.H. 1993. The chromosomal translocation t(X;14)(q28;q11) in T-cell pro-lymphocytic leukaemia breaks within one gene and activates another. Oncogene 8:3271-3276.
5. Fisch, P., Kohler, G., Garbe, A., Herbst, B., Wider, D., Kohler, H., Schaefer, H.E., Mertelsmann, R., Brugger, W., and Kanz, L. 1996. Generation of antigen-presenting cells for soluble protein antigens ex vivo from peripheral blood CD34+ hematopoietic progenitor cells in cancer patients. Eur J Immunol 26:595-600.
6. Fisch, P., Meuer, E., Pende, D., Rothenfusser, S., Viale, O., Kock, S., Ferrone, S., Fradelizi, D., Klein, G., Moretta, L., et al. 1997. Control of B cell lymphoma recognition via natural killer inhibitory receptors implies a role for human V9/V2 T cells in tumor immunity. Eur J Immunol 27:3368-3379.
7. Handgretinger, R., Geiselhart, A., Moris, A., Grau, R., Teuffel, O., Bethge, W., Kanz, L., and Fisch, P. 1999. Pure red-cell aplasia associated with clonal expansion of granular lymphocytes expressing killer-cell inhibitory receptors. N Engl J Med 340:278-284.
8. Fisch, P., Moris, A., Rammensee, H.G., and Handgretinger, R. 2000. Inhibitory MHC class I receptors on T cells in tumour immunity and autoimmunity. Immunol Today 21:187-191.
9. Rothenfusser, S., Buchwald, A., Kock, S., Ferrone, S., and Fisch, P. 2002. Missing HLA class I expression on Daudi cells unveils cytotoxic and proliferative responses of human T lymphocytes. Cell Immunol 215:32-44.
10. Ehl, S., Schwarz, K., Enders, A., Duffner, U., Pannicke, U., Kuhr, J., Mascart, F., Schmitt-Graeff, A., Niemeyer, C., and Fisch, P. 2005. A variant of SCID with specific immune responses and predominance of gamma delta T cells. J Clin Invest 115:3140-3148.
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