Tumorzentrum Freiburg - CCCF

Positions

• Medical Director of the Division of Pediatric Oncology and Hematology, Center for Paediatrics and Adolescent Medicine at the University of Freiburg
• Founding member of the study group „European Working Group of MDS in Childhood (EWOG-MDS), Chair of the Study Coordinating Center of EWOG-MDS
• Coordinating Investigator of the Society of Pediatric Oncology and Hematology in Germany/Austria/Switzerland (GPOH) for myelodysplastic syndromes (MDS) and  juvenile myelomonocytic leukemia (JMML)

Research Focus

• Genetics and therapy of Myelodysplastic Syndromes (MDS) in childhood
• Rasopathies and juvenile myelomonocytic leukemia (JMML)
• Secondary leukemia
• Genetics of Diamond Blackfan Anemia

Clinical Focus

• Myelodysplastic syndromes (MDS) in childhood: etiology and therapy
• Juvenile myelomonocytic leukemia (JMML)
• Inherited bone marrow failure
• Stem cell transplantation
• Coordinating study and reference center for myeloproliferative/-dysplastic neoplasia in pediatric patients  

DKTK Research Topic/Projects

Molecular diagnostics of pediatric malignancies
DNA Methylation Signatures and Response to Azacitidine Therapy in Juvenile Myelomonocytic Leukemia

Working Group

Group Leader    Prof. Christian Flotho
Group Leader    Prof. Jochen Roessler
Group Leader    Dr. Miriam Erlacher   
Group Leader    Dr. Marcin Wlodarski
Group Leader    Dr. Simone Hettmer
Group Leader    Dr. Brigitte Strahm    
Technical Laboratory Management       Dr. Thomas Gorr       
 

DKTK Publications

Speckmann C, Sahoo SS, Rizzi M, Hirabayashi S, Karow A, Serwas NK, Hoemberg M, Damatova N, Schindler D, Vannier J-B, Boulton SJ, Pannicke U, Göhring G, Thomay K, Verdu-Amoros JJ, Hauch H, Woessmann W, Escherich G, Laack E, Rindle L, Seidl M, Rensing-Ehl A, Lausch E, Jandrasits C, Strahm B, Schwarz K, Ehl SR, Niemeyer C, Boztug K, Wlodarski MW. Clinical and Molecular Heterogeneity of RTEL1 Deficiency. Frontiers in Immunology [Internet]. 1. Mai 2017 [zitiert 7. Juni 2017];8. Verfügbar unter: http://journal.frontiersin.org/article/10.3389/fimmu.2017.00449/full 

Wlodarski MW, Hirabayashi S, Pastor V, Stary J, Hasle H, Masetti R, Dworzak M, Schmugge M, van den Heuvel-Eibrink M, Ussowicz M, De Moerloose B, Catala A, Smith OP, Sedlacek P, Lankester AC, Zecca M, Bordon V, Matthes-Martin S, Abrahamsson J, Kuhl JS, Sykora K-W, Albert MH, Przychodzien B, Maciejewski JP, Schwarz S, Gohring G, Schlegelberger B, Cseh A, Noellke P, Yoshimi A, Locatelli F, Baumann I, Strahm B, Niemeyer CM, for the EWOG-MDS. Prevalence, clinical characteristics, and prognosis of GATA2-related myelodysplastic syndromes in children and adolescents. Blood. 17. März 2016;127(11):1387–97.

Wilhelm T, Lipka DB, Witte T, Wierzbinska JA, Fluhr S, Helf M, Mücke O, Claus R, Konermann C, Nöllke P, Niemeyer CM, Flotho C, Plass C. Epigenetic silencing of AKAP12 in juvenile myelomonocytic leukemia. Epigenetics. 18. Februar 2016;00–00. 

Pastor VB, Hirabayashi S, Karow A, Wehrle J, Kozyra EJ, Nienhold R, Ruzaike G, Lebrecht D, Yoshimi A, Niewisch M, Ripperger T, Göhring G, Baumann I, Schwarz S, Strahm B, Flotho C, Skoda RC, Niemeyer CM, Wlodarski MW. Mutational landscape in children with myelodysplastic syndromes is distinct from adults: Specific somatic drivers and novel germline variants. Leukemia [Internet]. 23. November 2016 [zitiert 5. Dezember 2016]; Verfügbar unter: http://www.nature.com/doifinder/10.1038/leu.2016.342

Krombholz CF, Aumann K, Kollek M, Bertele D, Fluhr S, Kunze M, Niemeyer CM, Flotho C, Erlacher M. Long-term serial xenotransplantation of juvenile myelomonocytic leukemia recapitulates human disease in Rag2-/- c-/- mice. Haematologica. 1. Mai 2016;101(5):597–606. 

Helsmoortel HH, De Moerloose B, Pieters T, Ghazavi F, Bresolin S, Cave H, de Vries A, de Haas V, Flotho C, Labarque V, Niemeyer C, De Paepe P, Van Roy N, Stary J, van den Heuvel-Eibrink MM, Benoit Y, Schulte J, Goossens S, Berx G, Haigh JJ, Speleman F, Van Vlierberghe P, Lammens T. LIN28B is over-expressed in specific subtypes of pediatric leukemia and regulates lncRNA H19. Haematologica. 1. Juni 2016;101(6):e240–4.

Fluhr S, Boerries M, Busch H, Symeonidi A, Witte T, Lipka DB, Mücke O, Nöllke P, Krombholz CF, Niemeyer CM, Plass C, Flotho C. CREBBP is a target of epigenetic, but not genetic, modification in juvenile myelomonocytic leukemia. Clinical Epigenetics [Internet]. Dezember 2016 [zitiert 11. Mai 2016];8(1). Verfügbar unter: http://clinicalepigeneticsjournal.biomedcentral.com/articles/10.1186/s13148-016-0216-3 

Botezatu L, Michel LC, Makishima H, Schroeder T, Germing U, Haas R, van der Reijden B, Marneth AE, Bergevoet SM, Jansen JH, Przychodzen B, Wlodarski M, Niemeyer C, Platzbecker U, Ehninger G, Unnikrishnan A, Beck D, Pimanda J, Hellström-Lindberg E, Cazzola M, Malcovati L, Boultwood J, Pellagatti A, Papaemmanuil E, Le Coutre P, Kaeda J, Opalka B, Möröy T, Dührsen U, Maciejewski J, Khandanpour C. GFI136N as a therapeutic and prognostic marker for myelodysplastic syndrome (MDS). Experimental Hematology [Internet]. April 2016 [zitiert 20. April 2016]; Verfügbar unter: http://linkinghub.elsevier.com/retrieve/pii/S0301472X16300649

Locatelli F, Niemeyer CM. How I treat juvenile myelomonocytic leukemia. Blood. 12. Februar 2015;125(7):1083–90.

Other Publications

Poetsch AR, Lipka DB, Witte T, Claus R, Nöllke P, Zucknick M, Olk-Batz C, Fluhr S, Dworzak M, De Moerloose B, Starý J, Zecca M, Hasle H, Schmugge M, van den Heuvel-Eibrink MM, Locatelli F, Niemeyer CM, Flotho C, Plass C. RASA4 undergoes DNA hypermethylation in resistant juvenile myelomonocytic leukemia. Epigenetics. 2014 Sep 1;9(9):1252-60. doi: 10.4161/epi.29941. Epub 2014 Jul 31. PubMed PMID: 25147919; PubMed Central PMCID: PMC4169017.  

Willasch AM, Kreyenberg H, Shayegi N, Rettinger E, Meyer V, Zabel M, Lang P, Kremens B, Meisel R, Strahm B, Rossig C, Gruhn B, Klingebiel T, Niemeyer CM, Bader P. Monitoring of Hematopoietic Chimerism after Transplantation for Pediatric Myelodysplastic Syndrome: Real-Time or Conventional Short Tandem Repeat PCR in Peripheral Blood or Bone Marrow? Biol Blood Marrow Transplant. 2014 Jul 31. pii: S1083-8791(14)00480-7. doi: 10.1016/j.bbmt.2014.07.030. [Epub ahead of print] PubMed PMID: 25087899.  

Lucena-Araujo AR, Kim HT, Jacomo RH, Melo RA, Bittencourt R, Pasquini R, Pagnano K, Fagundes EM, Chauffaille MD, Chiattone CS, Lima AS, Ruiz-Argüelles G,  Undurraga MS, Martinez L, Kwaan HC, Gallagher R, Niemeyer CM, Schrier SL, Tallman MS, Grimwade D, Ganser A, Berliner N, Ribeiro RC, Lo-Coco F, Löwenberg B, Sanz MA, Rego EM. Internal tandem duplication of the FLT3 gene confers poor overall survival in patients with acute promyelocytic leukemia treated with all-trans retinoic acid and anthracycline-based chemotherapy: an International Consortium on Acute Promyelocytic Leukemia study. Ann Hematol. 2014 Jul 2. [Epub ahead of print] PubMed PMID: 24981688.  

Lucena-Araujo AR, Kim HT, Jacomo RH, Melo RA, Bittencourt R, Pasquini R, Pagnano K, Fagundes EM, de Lourdes Chauffaille M, Chiattone CS, Lima AS, Kwaan HC, Gallagher R, Niemeyer CM, Schrier SL, Tallman MS, Grimwade D, Ganser A, Berliner N, Ribeiro RC, Lo-Coco F, Löwenberg B, Sanz MA, Rego EM. Prognostic impact of KMT2E transcript levels on outcome of patients with acute promyelocytic leukaemia treated with all-trans retinoic acid and anthracycline-based chemotherapy: an International Consortium on Acute Promyelocytic Leukaemia study. Br J Haematol. 2014 Aug;166(4):540-9. doi: 10.1111/bjh.12921. Epub 2014 May 3. PubMed PMID: 24796963.  

Aalbers, AM, van den Heuvel-Eibrink MM, Baumann I, Beverloo HB, Driessen GJ, Dworzak M, Fischer A, Zecca M, Zwaan CM, van Dongen JJ, Pieters R, Niemeyer CM, van der Velden VH, Langerak AW: T-cell receptor Vβ skewing frequently occurs in refractory cytopenia of childhood and is associated with an expansion of effector cytotoxic T-cells: a prospective study by EWOG-MDS Blood Cancer J, Epub2014 May 2;4:e209. doi: 10.1038/bcj.2014.28. PMID:24786393

Aalbers AM, van der Velden VH, Yoshimi A, Fischer A, Noellke P, Zwaan CM, Baumann I, Beverloo HB, Dworzak M, Hasle H, Locatelli F, De Moerloose B, Gohring G, Schmugge M, Stary J, Zecca M, Langerak AW, van Dongen JJ, Pieters R, Niemeyer CM, van den Heuvel-Eibrink MM: The clinical relevance of minor paroxysmal nocturnal hemoglobinuria clones in refractory cytopenia of childhood: a prospective study by EWOG-MDS. Leukemia, 2014, 28: 189-192. doi: 10.1038/leu.2013.195. Epub 2013 Jun 28 PMID:23807769

Becker H, Yoshida K, Blagitko-Dorfs N, Claus R, Pantic M, Abdelkarim M, Niemöller C, Greil C, Hackanson B, Shirashi Y, Chiba K, Tanaka H, Miyano S, Döhner K, Schnittger S, Henneke P, Niemeyer CM, Flotho C, Pfeifer D, Ogawa S, Lübbert M: Tracing the development of acute myeloid leukemia in CBL syndrome. Blood, 2014; 123 (12): 1883-1886. doi: 10.1182/blood-2013-10-533844.Epub 2014 Feb 3. PMID:24493670

Campagna DR, de Bie CI, Schmitz-Abe K, Sweeney M, Sendamarai AK, Schmidt PJ, Heeney MM, Yntema HG, Kannengiesser C, Grandchamp B, Niemeyer CM, Knoers NV, Swart S, Marron G, van Wijk R, Raymakers RA, May A, Markianos K, Bottomley SS, Swinkels DW, Fleming MD: X-linked sideroblastic anemia due to ALAS2 intron 1 enhancer element GATA-binding site mutations. Am J Hematol, 2014; 89 (3): 315-319  

Yoshimi A, Strahm B, Baumann I, Furlan I, Schwarz S, Teigler-Schlegel A, Walther JU, Schlegelberger B, Göhring G, Nöllke P, Führer M, Niemeyer CM: Hematopoietic stem cell transplantation in children and young adults with secondary myelodysplastic syndrome and acute myelogenous leukemia after aplastic anemia. Biol Blood Marrow Tr, 2014; 20: 421-434. doi: 10.1016/j.bbmt.2013.11.031. Epub 2013 Dec 5. PMID:24316460

Yoshimi A, van den Heuvel-Eibrink MM, Baumann I, Schwarz S, Simonitsch-Klupp I, de Paepe P, Campr V, Birk Kerndrup G, O' Sullivan M, Devito R, Leguit R, Hernandez M, Dworzak M, de Moerloose B, Stary J, Hasle H, Smith OP, Zecca M, Catala A, Schmugge M, Locatelli F, Fuhrer M, Fischer A, Guderle A, Nollke P, Strahm B, Niemeyer CM: Comparison of horse and rabbit anti-thymocyte globulin in immunosuppressive therapy for refractory cytopenia of childhood. Haematol-hematol J, 2014; 99: 656-663. doi: 10.3324/haematol.2013.095786. Epub 2013 Oct 25. PMID:24162791