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Tumorzentrum Freiburg - CCCF

Prof. Dr. Sven Diederichs

Prof. Dr. Sven Diederichs
Division of Cancer Research
Department  of Thoracic Surgery
Center for Translational Cell Research (ZTZ)
Breisacher Str. 115
D-79106 Freiburg
Phone: +49 (0) 761 270 77571
lung-cancer-research@uniklinik-freiburg.de

Current position:

Division Head - Division of Cancer Research, Department of Thoracic Surgery
Website:  Division of Cancer Research

Research Focus:

The research of the division focuses on long non-coding RNAs (lncRNAs). Based on expression profiling using microarrays as well as deep RNA sequencing of the whole transcriptome, this group elucidates the cellular and molecular functions of differentially regulated ncRNAs in cancer using innovative techniques like the CRISPR/Cas9 system, in vivo RNA Affinity Purification and own customized siRNA and CRISPR libraries targeting specifically lung cancer-associated lncRNAs.  RNAi screens have uncovered numerous lncRNAs controlling several hallmarks of cancer including cancer cell viability, mitosis and migration.

DKTK Research Topic:

T4 Oncogenic Signaling
Project: Oncogenic Pathways in Lung Cancer & Metastasis

CRC 850 Project:

The function of long non-coding RNAs in lung cancer cell migration and metastasis

DKTK Publications

S Dhamija*, CM Yang*, J Seiler, K Myacheva, M Caudron-Herger, A Wieland, M Abdelkarim, Y Sharma, M Riester, M Groß, J Maurer, S Diederichs: "A Pan-Cancer Analysis reveals Nonstop Extension Mutations causing SMAD4 Tumor Suppressor Degradation"
Nature Cell Biology (2020) epub

M Gandhi, M Groß, JM Holler, SA Coggins, N Patil, JH Leupold, M Munschauer, M Schenone, CR Hartigan, H Allgayer, B Kim, S Diederichs: "The lncRNA lincNMR regulates nucleotide metabolism via a YBX1 - RRM2 axis in cancer"
Nature Communications (2020) 11: 3214

M Caudron-Herger, E Wassmer, I Nasa, AS Schultz, J Seiler, AN Kettenbach, S Diederichs: "Identification, quantification and bioinformatic analysis of RNA-dependent proteins by RNase treatment and density gradient ultracentrifugation using R-DeeP"
Nature Protocols (2020) 15: 1338-1370

Y Sharma*, M Miladi*, S Dukare*, K Boulay*, M Caudron-Herger, M Groß, R Backofen, S Diederichs: "A pan-cancer analysis of synonymous mutations"
Nature Communications (2019) 10: 2569

M Caudron-Herger, SF Rusin, ME Adamo, J Seiler, V Schmid, E Barreau, AN Kettenbach, S Diederichs: "R-DeeP: Proteome-wide and Quantitative Identification of RNA-dependent Proteins by Density Gradient Ultracentrifugation"
Molecular Cell (2019) 75: 184-199

Nötzold L, Frank L, Gandhi M, Polycarpou-Schwarz M, Groß M, Gunkel M, Beil N, Erfle H, Harder N, Rohr K, Trendel J, Krijgsveld J, Longerich T, Schirmacher P, Boutros M, Erhardt S, Diederichs S. The long non-coding RNA LINC00152 is essential for cell cycle progression through mitosis in HeLa cells. Scientific Reports. Dezember 2017 [zitiert 7. Juni 2017];7(1).

Klingenberg M, Matsuda A, Diederichs S, Patel T. Non-coding RNA in hepatocellular carcinoma: Mechanisms, biomarkers and therapeutic targets. Journal of Hepatology. April 2017 [zitiert 3. Mai 2017]

Das K, Eisel D, Lenkl C, Goyal A, Diederichs S, Dickes E, Osen W, Eichmüller SB. Generation of murine tumor cell lines deficient in MHC molecule surface expression using the CRISPR/Cas9 system. Fujii H, Herausgeber. PLOS ONE. 16. März 2017;12(3):e0174077.

Sas‐Chen A, Aure MR, Leibovich L, Carvalho S, Enuka Y, Körner C, Polycarpou‐Schwarz M, Lavi S, Nevo N, Kuznetsov Y, Yuan J, Azuaje F, Ulitsky I, Diederichs S, Wiemann S, Yakhini Z, Kristensen VN, Børresen‐Dale A, Yarden Y. LIMT is a novel metastasis inhibiting lncRNA suppressed by EGF and downregulated in aggressive breast cancer. EMBO Molecular Medicine. 1. August 2016;e201606198.

Nöske K, Stark H-J, Nevaril L, Berning M, Langbein L, Goyal A, Diederichs S, Boukamp P. Mitotic Diversity in Homeostatic Human Interfollicular Epidermis. International Journal of Molecular Sciences. 28. Januar 2016;17(2):167.

Link S, Grund SE, Diederichs S. Alternative splicing affects the subcellular localization of Drosha. Nucleic Acids Research. 16. Mai 2016;gkw400.

Goyal A, Myacheva K, Groß M, Klingenberg M, Duran Arqué B, Diederichs S. Challenges of CRISPR/Cas9 applications for long non-coding RNA genes. Nucleic Acids Research. 30. September 2016;gkw883.

Diederichs S, Bartsch L, Berkmann JC, Fro se K, Heitmann J, Hoppe C, Iggena D, Jazmati D, Karschnia P, Linsenmeier M, Maulhardt T, Mo hrmann L, Morstein J, Paffenholz SV, Ro penack P, Ru ckert T, Sandig L, Schell M, Steinmann A, Voss G, Wasmuth J, Weinberger ME, Wullenkord R. The dark matter of the cancer genome: aberrations in regulatory elements, untranslated regions, splice sites, non-coding RNA and synonymous mutations. EMBO Molecular Medicine [Internet]. 18. März 2016 [zitiert 11. April 2016]; Verfügbar unter: embomolmed.embopress.org/cgi/doi/10.15252/emmm.201506055

Diaz-Lagares A, Crujeiras AB, Lopez-Serra P, Soler M, Setien F, Goyal A, Sandoval J, Hashimoto Y, Martinez-Cardús A, Gomez A, Heyn H, Moutinho C, Espada J, Vidal A, Paúles M, Galán M, Sala N, Akiyama Y, Martínez-Iniesta M, Farré L, Villanueva A, Gross M, Diederichs S, Guil S, Esteller M. Epigenetic inactivation of the p53-induced long noncoding RNA TP53 target 1 in human cancer. Proceedings of the National Academy of Sciences. 7. November 2016;201608585.

Dhamija S, Diederichs S. From junk to master regulators of invasion: lncRNA functions in migration, EMT and metastasis: lncRNAs in Metastasis. International Journal of Cancer. 15. Juli 2016;139(2):269–80.

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Prof. Dr. Christoph Peters
Tumorzentrum Freiburg CCCF
Phone: +49-761-270-71500
Hugstetter Str. 49
79106 Freiburg

and

Institute of Molecular Medicine and Cell Research
University of Freiburg
Stefan-Meier-Str. 17
79104 Freiburg
Phone: +49-761-203-9600/9601
christoph.peters@mol-med.uni-freiburg.de​​

Dr. Anja Hernández
Deutsches Krebsforschungszentrum (DKFZ)
Im Neuheimer Feld 280
69120 Heidelberg
++49 (0)761 270 22810
anja.hernandez@dkfz-heidelberg.de

Dr. Anna Dost
Deutsches Krebsforschungszentrum (DKFZ)
Im Neuheimer Feld 280
69120 Heidelberg
+49 (0)761 270 77121
a.dost@dkfz-heidelberg.de