Overview Flow Cytometry
Cell Sorting and FACS Analysis
Cell Sorting
Lighthouse operates three high speed cell sorters: a 16-color FACS Aria Fusion and a 17-color FACS Aria III, both from Becton Dickinson, as well as a 14-color MoFlo Astrios EQ cell sorter from Beckman Coulter. Due to their complexity these machines must be operated by Lighthouse staff members.
More information about Cell Sorting
FACS Analysis
For FACS analysis we have a number of machines with different lasers and possible combinations of detectable colors.
More information about FACS Analysis
Offline Analysis
Cloud Licenses
Lighthouse now has a FluoroFinder Academic Plus Sitewide License. Fluorofinder has many useful tools to help you get started with panel design, in addition to a spectra viewer, validated panels, and directories of antibodies and fluorophores. All Lighthouse cytometers (analyzers and sorters) are listed on Fluorofinder, to make it easier for our users to develop panels for their experiments. Access for all is free, with more advanced tools available under the Sitewide license (for example AI Intellipanel tool). If you would like access to the Lighthouse Academic Plus Sitewide license, please contact us at FACSlab@uniklinik-freiburg.de and we will have your account activated.
Lighthouse also has a Cytobank Premium Cloud License, with many tools for dimensionality reduction, correction of batch effects and other useful tools for high-end cytometry analysis. All Lighthouse users can use the license. To get access to Cytobank, please contact us at FACSlab@uniklinik-freiburg.de and we will have your account activate.
Lighthouse also has offline analysis workstations with several flow cytometry analysis software programs that you are welcome to use:
Offline Licenses
FlowJo
Kaluza
ModFit
FACSArray
SONY ID7000 Analysis Software