Welcome to the Light Imaging Facility at IMITATE
The Bio Imaging Core Light Microscopy (BiMiC) is an open platform that enables researchers to perform leading-edge microscopy. The Platform aims to advance optical imaging throughout the Medical Faculty of the University of Freiburg. It is a major tool in the bench-to-patient back-and-forth research for novel therapies. The platform is located at the Institute for Disease Modeling and Targeted Medicine (IMITATE) where it plays a decisive role in the integrated multimodal imaging strategy of the institute.
The BiMiC is paired with the Lighthouse Core Facility offering a range of cutting-edge microscopes for imaging of live and fixed samples from various model organisms (Xenopus, Zebrafisch, C. elegans and Drosophila) and patients.
The BiMiC has a variety of tools and knowledgeable experts ready to help and guide research teams in all areas of optical imaging, from education to the creation of analysis pipelines.
Bio Imaging Core Light Microscopy (BiMiC)
Medical Faculty
Institute for Disease Modeling and Targeted Medicine (IMITATE)
Uniklinik Freiburg
Breisacherstrasse 113
79106 Freiburg
Tel. +49 (0) 761 270 63213
Email: franck.ditengou(at)uniklinik-freiburg.de
The BiMiC is paired and benefit from the support of the Lighthouse Core Facilty led by Dr. Marie Follo, and is embbeded in the The Microscopy and Image Analysis Platform (MIAP) headed by Dr. Roland Nitschke.
Zeiss Axioscan 7
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Efficient slide scanner for polarization, bright field, and fluorescence imaging. It can scan up to 100 slides with various profiles and imaging modes in a single pass. The system is a part of the ZEN imaging software ecosystem, giving users access to a wide range of extra processing and analysis capabilities. |
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Filter sets • 38 HE GFP shift free • 43 HE Cy3 shift free • 50 Cy5 shift free • 64 HE mPlum shift free (ex.587/em.647) • 96 HE BFP • 112 SBP (DAPI-GFP-dsRed-Cy5-Cy7) • FL EC ACR • OPO dyes |
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Objectives: • Fluar 5x/0.25 • PApo 10x/0,45 • PApo 20x/0,8 • EC Plan-NEOFLUAR 20x/0,5 • Plan-Apochromat 40x/0,95 Korr |
Zeiss Cell discoverer 7 with LSM 900 Airyscan Confocal
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High-end imaging fully integrated system offering a range of incubation and detection choices. The Zeiss CD7 combines traditional inverted microscope picture quality and flexibility with the user-friendly automation of a boxed microscope. Celldiscoverer 7 adjusts the optics automatically, calibrates itself, and detects and focuses your samples. |
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• Live Cell Imaging of 2D or 3D cell culture - full climate chamber • XY Motor - multi well / multipositional time series, tiled scans • 32 channel spectral scanning - fast, multicolor imaging • Zen Blue (widefield and confocal) • AiryScan detector / AiryScan Fast |
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Filtersets • 90 HE • 91 HE • 92 HE |
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Objectives • PApo 20x/0,7 autocorr • PApo 20x/0,95 autocorr • PApo 50x/1,2W m/Autoimmersion |
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Illumination • Colibri.2 extension |
ZEISS LIGHTSHEET 7.1
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This system is ideal for imaging full living model organisms, tissues, and cells quickly and gently with minimal photo toxicity as they evolve over time in a controlled temperature and CO2 condition. |
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Objectives • 5x/0,16 foc PApo • 10x0,5 (UV) VIS-NIR Water immersion • Plan-APO 20x/1,0 DIC Water immersion • Plan-APO 63x/1,0 Clr PN 20x/1,0 Korr nd=1,45 |
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Laser lines • 405nm (DAPI, Alexa 405, Brilliant Violets) • 488 nm (Alexa 488, EGFP, FITC, CY2) • 561nm (CY3, TRITC, Alexa 555/568, PI, mCherry, Texas Red) • 633nm laser (CY5, Alexa 633 / 647, DRAQ-5/7) |
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Filters • BP 420-470 + BP 505-545 • BP 505-545 + LP 585 • BP 505-545 + LP 660 • BP 575-615 + LP 660 |
Zeiss LSM 980 Airyscan2 with multiphoton excitation
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Confocal 4D imaging platform with optical sectioning, minimal phototoxicity, and quick processing. Two photon excitation microscopy allows imaging relatively thick samples (up to about 1 mm thickness). Ideal for photoconversion and tissue ablation. |
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Laser lines available • 405nm (DAPI, Alexa 405, Brilliant Violets) • 488 nm (Alexa 488, EGFP, FITC, CY2) • 561nm (CY3, TRITC, Alexa 555/568, PI, mCherry, Texas Red) • 633nm laser (CY5, Alexa 633 / 647, DRAQ-5/7) |
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Emission Filters • BP 420-470 + BP 505-545 • BP 505-545 + LP 585 • BP 505-545 + LP 660 • BP 575-615 + LP 660 • NDD Filterset 3i IR+ blue/green/red • NDD Modul D 2Ch (0°) HS f. invers • NDD Filterset 1i IR+ blue/green • BiG-Filterset 2 BiG+ blue/green • BiG-Filterset 4 BiG+ green/red |
Online booking
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Registration required |
Downloads
Axioscan quick hands on
CD7 system start and stop
Lightsheet start and stop
