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Klinik für Innere Medizin IHämatologie, Onkologie und Stammzelltransplantation

Lighthouse Core Facility

 

 

The Lighthouse Core Facility was founded to give investigators access to a broad spectrum of fluorescence-based technologies useful in answering research questions in the areas of cell and molecular biology. For more information about what instruments and services are available at the facility and how you can take advantage of what we have to offer, click on the appropriate sections below.

                                   

 

Sorter Booking Online

We are in the process of integrating a completely new booking and calendar system from Booked Scheduler for the Lighthouse Core Facility. The cell sorting calendars have already been migrated, with the remaining machines set to follow within the next few weeks.

For the cell sorters, you can use the new calendar to look online at current bookings to find an available sorting slot, and you can even request a specific sort time by provisionally reserving a sort appointment in advance. If the requested appointment is accepted, you will receive a written confirmation of the desired appointment. Only then is your sort appointment considered "officially" to be scheduled.

Our new calendar system can be easily accessed, even from outside the Uniklinik network via the Internet. However, to access the system you first have to register as a user in it. For users with an Uniklinik LDAP login the registration process is very straightforward. Using your LDAP login and the corresponding password you can log in. This will automatically send us a request to activate your account. Once your user account has been activated, you will receive a one-time confirmation email from us. After that, you can then use the system.

External users (Vorklinik, University) can also access the system. To do so, please send a request to facslab(ät)uniklinik-freiburg.de.

Currently, only the cell sorting calendars are active, but users will soon be able to access calendars for all of our other systems once they also go online.

The address of the new Lighthouse booking system can be found at the following link: https://ztz-buchung.uniklinik-freiburg.de/Web/index.php (or just booking.core-facility.de)

 

COVID-19 / SARS-CoV2 / Corona Information

Lighthouse Core Facility Rules & Measures

Due to the spread of the SARS-CoV2/coronavirus, access to the Lighthouse Core Facility is currently limited.

Training sessions for new users are limited due to the current situation.

You can email us (facslab(ät)uniklinik-freiburg.de) about signing up for the next available training session.

Experienced users are allowed to continue to use our equipment but only when following these rules and restrictions:

 

Rules for Operating Lighthouse Core Facility Equipment During the SARS-CoV-2 Pandemic

  • In agreement with government guidelines with regard to enclosed public spaces, we ask that all users wear masks while working within the rooms of the Lighthouse Core Facility (see details below).
  • Only registered (and experienced) users are allowed to use our equipment.
  • A minimum distance of 1,5 meters between users must be maintained all times.
  • Each machine may be used by only one user at a time.
  • Disinfectant is located in every bay of the main lab, and in every imaging room, and we urge you to use it to disinfect the computer keyboard, mouse and equipment controls before using the equipment.
  • Users who are SARS-CoV-2 positive or otherwise sick are not allowed to access the facility even if they appear to have only mild cold-like or other similar symptoms.

 

1. Important hygiene measures upon entering the Lighthouse Core Facility

  • At the main entrance of lab 009: Wash or disinfect your hands!
  • All users have to wear fresh gloves! So put gloves on, if you need replacements you can find them in the room on the table (size M only).
  • Do not touch bare surfaces with bare hands (i.e. door handles).
  • All users from labs at ZTZ have to bring their own fresh lab coats. Users from outside of ZTZ please use a fresh coat provided by the Lighthouse Core Facility. Put the coat on while entering the facility. Take your own coat with you after your session, for repeated coat usage ask the Lighthouse staff on site. Lab coats provided by Lighthouse Core Facility have to be left at the end of your session in the laundry bag beside the lab's main entrance or at the Lighthouse lockers!
  • All users must bring a personal or single-use face mask with them! If you have forgotten them, the Lighthouse Core Facility currently has no face masks available and you are not allowed to work!

 

2. Additional important hygiene measures in the microscope rooms

  • All users must bring eye protecting safety goggles and a personal or single-use face mask with them! If you forget them, you are not allowed to work!
  • Never work without gloves, if you need replacements you can find them in the room on the table (size M only).
  • Cover all touchable surfaces such as keyboard, mouse, eyepieces and binocular microscope body with new plastic wrap (cling film / Frischhalte-folie).
  • More instructions  for the end of your session are located within each microscope room.

 

Detailed Picture-Story on “How to wrap” the equipment are in the microscope rooms.

Note!: If the user before you left the plastic wrap on the microscope, you still have to exchange it before your session.

 

3. Important hygiene measures when leaving the Lighthouse Core Facility Area

• Last step at the main exit of lab 009: Take off the lab coat, leave ours in the laundry bag, take yours with you. Take off your gloves carefully, throw them into trash, and wash your hands or use the hand disinfectant.

These measures are valid until further notice.

IncuCyte S3 out of order

Unfortunately the IncuCyte needs a repair in England. We expect the microscope back somewhat in the middle of May.

SONY Training on 30.07.2020

A basic user introduction for the Sony FACS Analyzer will take place in the Lighthouse Core Facility.

The event is limited to a max. no. of 2 participants. The introduction can be in German or English. If you are interested please email us for a binding reservation.

Where: Lighthouse Core Facility, ZTZ, ground floor, room 009, bay 3 at the Sony Analyzer

When: 30.07.2020 (from 9.00 AM until 11.30 AM)

Visiting any introduction is free of charge and required before using the Sony Analyzer at the Lighthouse Core Facility.

Please bring pen and paper for your notes in any case and your own lab coat if you are based at ZTZ.

Note: The number of participants is limited and the registration is binding after confirmation by us. If you are unable to attend, please let us know as soon as possible. So there is still a chance for substitutes.

More information about the course.

Flow Cytometry

Cell Sorting

Available on Becton Dickinson's FACSAria III and FACSAria Fusion and Beckman Coulter's MoFlo Astrios EQ.
More...

Laser beams in a Fortessa

FACS Analysis

Available on Becton Dickinson's LSR Fortessas, Canto, Beckman Coulter's Gallios & Navios, and Sony's Spectral Analyser SP6800.
More...

Microscopy
Green, red and blue fluorescence in a cell

Confocal Microscopy

For confocal microscopy we have microscopes from Zeiss (LSM 880 with AiryScan) and LSM 710 and Leica's TCS SP2 AOBS available.
More...

Long-Term Live Cell Imaging

The Incucyte S3 is an automated microscope located within a standard cell culture incubator and is of particular interest for low resolution, long-term, live cell imaging. It allows the independent measurement and analysis of up to 6 plates or experiments at the same time. It is the method of choice for long-term observation of spheroid and organoid development.

More information about Incucyte long-term live cell imaging...

High Content Screening / Image Cytometry

Lighthouse has an Olympus Scan^R High Content Screening Station which allows automated image acquisition and data analysis of biological samples. Other terms for this field include image cytometry or automated microscopy.
More...

Nucleic Acid Analysis

Quantitative PCR

Lighthouse has two Roche LightCycler 480 (LC 480) machines for quantitative PCR analysis. Each LC 480 is a plate-based machine with both 384-well and 96-well blocks. The thermal blocks are fast-cycling, with typical run times slightly under one hour. The range of reaction volumes is 20 - 100 microliters for the 96-well and 5 - 20 microliters for the 384-well block.
More...

Droplet Digital PCR

In addition ti its Bio-Rad QX100 Droplet Digital PCR (ddPCR) system Lighthouse has just added a Stilla Naica Crystal Digital PCR system.
More...

Our Network

MIAP

Lighthouse is a member unit of the Microscopy and Image Analysis Platform (MIAP), an international network of microscopy and image analysis facilities from throughout the region, including Freiburg, Basel and Strasbourg.

Overview of MIAP Partner Units

Cytometry.de

Lighthouse is a part of cytometry.de, a national network of cytometrists and cytometry core facilities of the DGfZ / Deutsche Gesellschaft für Zytometrie.

RIsources

Lighthouse is listed within the RIsources Database of the DFG. The RIsources listing simplifies applying for funding for the use of Lighthouse services within DFG grant applications.

Lighthouse RIsources Page

Please note:

Our user fees cover only a small fraction of our total running costs, most of which in fact are covered by our home institutes (Klinik für Innere Medizin I, Centrum für Chronische Immundefizienz (CCI), Tumorzentrum Freiburg/DKTK (CCCF). The majority of our machines were financed with the help of outside sources such as the DFG, Land Baden-Württemberg, or BMBF. We therefore depend on proper acknowledgement in publications, grants and grant applications. Publications, posters or presentations that make use of services or data collected at the facility or by its staff should list these contributions accordingly. Thank you.

If you still have questions about the facility after reading through these pages, feel free to contact us at one of the numbers listed on the right side.

 

Universitätsklinikum Freiburg
Zentrum für Translationale Zellforschung (ZTZ)
Department of Medicine I
Tumorzentrum Freiburg - CCCF/DKTK
Center for Chronic Immune Deficiency - CCI

Contact | Information

 

FACS-Sorting Booking:

Email:

Online Booking Calendar (new!)
Facslab-email

 

Online Reservations (for machines others than cell sorters):

Booking Calendar (intranet only)
 

Head of Facility:

Marie Follo, Ph.D.
Telephone: 0761 270-77697
Telefax: 0761 270-63780
Email: Marie Follo

 

Address:

Universitätsklinikum Freiburg
Lighthouse Core Facility

Zentrum für Translationale Zellforschung (ZTZ)
Breisacher Straße 115
D-79106 Freiburg

www.core-facility.de