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Klinik für Innere Medizin IHämatologie, Onkologie und Stammzelltransplantation

Lighthouse Core Facility

 

 

 

The Lighthouse Core Facility was founded to give investigators access to a broad spectrum of fluorescence-based technologies useful in answering research questions in the areas of cell and molecular biology. For more information about what instruments and services are available at the facility and how you can take advantage of what we have to offer, click on the appropriate sections below.

November is "Microscopy Month" at Lighthouse

During the month of November we will be hosting 3  different workshops for 3 very different types of systems/approaches to using microscopy in the life sciences which Lighthouse would like to invite you to attend.

The first workshop is on High-Content Imaging and Analysis and will take place November 12-14.

There will be a seminar discussing the approach on the morning of November 12, with chances to test and analyse your samples directly on a Perkin Elmer Operetta CLS system after that. This is a spinning disk system able to examine 3D objects or structures such as spheroids or organoids. It is also able to examine tissue sections.

MIAP HCS Workshop

Perkin Elmer Operetta CLS

November 12-14, 2019

Seminar Information:

Date/Time: Nov. 12, 2019, 9:00 - 10:30 am

Location: ZTZ, EG Seminarraum Links, 00.057

Title: The power of high-content imaging and analytics to illuminate cell population dynamics

Speaker: Dr. Achim Kirsch, Application Scientist HCS

Workshop timeslots on the Operetta are available from Nov. 12, 10:30 am until Nov. 14, 12:00 am. To reserve a slot or for more information please contact: facslab@uniklinik-freiburg

Download detailed information here  

ZEISS Workshop

Next Generation Multiplex Confocal Imaging with the New ZEISS LSM 9 Family

November 19-21, 2019

You are cordially invited to the Workshop “Next Generation Multiplex Confocal Imaging“ in which we would like to introduce you to brand new developments in live cell imaging.

Discover the ideal confocal microscope platform for 4D imaging with new ZEISS LSM 980 and ZEISS LSM 900 combined with the new Multiplex mode for parallel pixel acquisition on Airyscan 2. Convince yourself in a comprehensive workshop of lectures and system live demonstrations how your work can benefit from:

  • Multiplex mode for parallel pixel acquisition acquires up to 8 superresolution image lines with high a signal-to-noise ration (SNR) in a single sweep.
  • ZEN imaging software with Smart Setup. Sample Navigator, and Direct Processing makes your work more efficient.
  • A light efficient beam path and Airyscan 2 offer a unique combination of gentle superresolution and high SNR.

Check out the innovations in multiplex imaging directly on site in individual handson sessions.

Register now at ZEISS - limited places available

We are looking forward to welcoming you at the Workshop!

Program

Workshop 1st Day | November 19

12.45 pm Registration
1.00 pmWelcome
1.15 pmThe New LSM 9 Family with Airyscan 2 - Adding the Multiplex Factor to Your Confocal Imaging
Andreas Lutter & Gert Sonntag, Imaging Specialists ZEISS Microscopy
2.00 pmCoffee and Discussion
2.30 pmSystem Live Demonstration: LSM 900 Airyscan 2
3.30 pmEnd

 

Workshop 2nd Day | November 20

Training Konfokale Mikroskopie (Deutsch)

10.00 amWillkommen
10.15 amKonfokale Mikroskopie: Grundprinzipien - der schnelle Weg zum ersten Bild
11.15 amKaffeepause
11.30 amBildaufnahme I: Aufnahmeparameter optimieren - Konfokale Detektion
12.30 pmMittagspause
1.15 pmBildaufnahme II: Mehrdimensionale Bildaufnahme: Z-Stapel, Zeitserien, Kacheln, Positionen, Fokusstrategien
2.15 pmKaffeepause
2.30 pmAiryscanning: Grundprinzipien - Airyscan FAST - Multiplexing
3.30 pmKaffeepause
3.45 pmMultichannel Unmixing - Photomanipulation - Prozessierung
4.45 pmEnde

 

 

Workshop 3rd Day | November 21

Training Confocal Microscopy (English)

10.00 amWelcome
10.15 amConfocal Microscopy: Basic Principles - The First Frames
11.15 amCoffee break
11.30 amImage Acquisition I - Optimizing imaging parameters - detectors
12.30 pmLunch
1.15 pmImage Acquisition II: Multidimensional Acquisition: z-stacks, time series, tiles, focus strategies
2.15 pmCoffee break
2.30 pmAiryscanning: Basic principles - Airyscan FAST - Multiplexing
3.30 pmCoffee break
3.45 pmMultichannel Unmixing - Photo-Manipulation - Processing
4.45 pmEnd

 

Venue

Universitätsklinikum Freiburg
Lighthouse Core Facility - Lecture Hall 00.057
Zentrum für Translationale Zellforschung - Klinik für Innere Medizin I
Breisacher Straße 115
79106 Freiburg
Germany

Lighthouse Core Facility Logo

Spectral Analyzer from SONY

Our latest flow cytometer is a SONY SP6800 ZE Spectral Analyzer. It has a novel spectral detector, allowing the unmixing (separation) and analysis of multiple, even spectrally overlapping fluorochromes, simultaneously.

FACS Fortessa Introduction 05.11.2019

The basic user introduction on FACS Analyser "Fortessa" takes place in the Core Facility. The event is limited to a maximum number of 4 participants. The introduction could be in English on request. If you are interested please contact us by email for a binding reservation. Please let us know your name, lab, phone number and the date of the training. We will inform you.

Where: Lighthouse Core Facility, ZTZ, ground floor, room 009, bay 3 at Fortessa "Leonardo"

When: 5. November 2019 from 9.00 AM to 11.30 AM

Visiting of the introduction is free of charge and required for using our Fortessas at Lighthouse Core Facility. Please bring pen and paper for your notes in any case and your own lab coat if you are based at ZTZ.

Note: The number of participants is limited and the registration is binding after confirmation by us. If you are unable to attend, please let us know as soon as possible. So there is still a chance for substitutes.

More information about the course.

SONY Training 13.11.19

A basic user introduction for the Sony FACS Analyzer will take place in the Lighthouse Core Facility.

The event is limited to a max. no. of 5 participants. The introduction will be in English. If you are interested please email us for a binding reservation.

Where: Lighthouse Core Facility, ZTZ, ground floor, room 009, bay 3 at the Sony Analyzer

When:  13. November 2019 (from 9.00 AM until 11.30 AM)

Visiting any introduction is free of charge and required before using the Sony Analyzer at the Lighthouse Core Facility.

Please bring pen and paper for your notes in any case and your own lab coat if you are based at ZTZ.

Note: The number of participants is limited and the registration is binding after confirmation by us. If you are unable to attend, please let us know as soon as possible. So there is still a chance for substitutes.

More information about the course.

FACS Fortessa Introduction 18.11.2019

The basic user introduction on FACS Analyser "Fortessa" takes place in the Core Facility. The event is limited to a maximum number of 4 participants. The introduction could be in English on request. If you are interested please contact us by email for a binding reservation. Please let us know your name, lab, phone number and the date of the training. We will inform you.

Where: Lighthouse Core Facility, ZTZ, ground floor, room 009, bay 3 at Fortessa "Leonardo"

When: 18. November 2019 from 9.00 AM to 11.30 AM

Visiting of the introduction is free of charge and required for using our Fortessas at Lighthouse Core Facility. Please bring pen and paper for your notes in any case and your own lab coat if you are based at ZTZ.

Note: The number of participants is limited and the registration is binding after confirmation by us. If you are unable to attend, please let us know as soon as possible. So there is still a chance for substitutes.

More information about the course.

FACS Fortessa Introduction 02.12.2019

The basic user introduction on FACS Analyser "Fortessa" takes place in the Core Facility. The event is limited to a maximum number of 4 participants. The introduction could be in English on request. If you are interested please contact us by email for a binding reservation. Please let us know your name, lab, phone number and the date of the training. We will inform you.

Where: Lighthouse Core Facility, ZTZ, ground floor, room 009, bay 3 at Fortessa "Leonardo"

When: 2. December 2019 from 9.00 AM to 11.30 AM

Visiting of the introduction is free of charge and required for using our Fortessas at Lighthouse Core Facility. Please bring pen and paper for your notes in any case and your own lab coat if you are based at ZTZ.

Note: The number of participants is limited and the registration is binding after confirmation by us. If you are unable to attend, please let us know as soon as possible. So there is still a chance for substitutes.

More information about the course.

SONY Training on 11.12.2019

A basic user introduction for the Sony FACS Analyzer will take place in the Lighthouse Core Facility.

The event is limited to a max. no. of 5 participants. The introduction will be in English. If you are interested please email us for a binding reservation.

Where: Lighthouse Core Facility, ZTZ, ground floor, room 009, bay 3 at the Sony Analyzer

When: 11. December 2019 (from 9.00 AM until 11.30 AM)

Visiting any introduction is free of charge and required before using the Sony Analyzer at the Lighthouse Core Facility.

Please bring pen and paper for your notes in any case and your own lab coat if you are based at ZTZ.

Note: The number of participants is limited and the registration is binding after confirmation by us. If you are unable to attend, please let us know as soon as possible. So there is still a chance for substitutes.

More information about the course.

FACS Fortessa Introduction 16.12.2019

The basic user introduction on FACS Analyser "Fortessa" takes place in the Core Facility. The event is limited to a maximum number of 4 participants. The introduction could be in English on request. If you are interested please contact us by email for a binding reservation. Please let us know your name, lab, phone number and the date of the training. We will inform you.

Where: Lighthouse Core Facility, ZTZ, ground floor, room 009, bay 3 at Fortessa "Leonardo"

When: 16. December 2019 from 9.00 AM to 11.30 AM

Visiting of the introduction is free of charge and required for using our Fortessas at Lighthouse Core Facility. Please bring pen and paper for your notes in any case and your own lab coat if you are based at ZTZ.

Note: The number of participants is limited and the registration is binding after confirmation by us. If you are unable to attend, please let us know as soon as possible. So there is still a chance for substitutes.

More information about the course.

Flow Cytometry

Cell Sorting

Available on Becton Dickinson's FACSAria III and FACSAria Fusion and Beckman Coulter's MoFlo Astrios EQ.
More...

Laser beams in a Fortessa

FACS Analysis

Available on Becton Dickinson's LSR Fortessas, Canto, Beckman Coulter's Gallios & Navios, and Sony's Spectral Analyser SP6800.
More...

Microscopy
Green, red and blue fluorescence in a cell

Confocal Microscopy

For confocal microscopy we have microscopes from Zeiss (LSM 880 with AiryScan) and LSM 710 and Leica's TCS SP2 AOBS available.
More...

Long-Term Live Cell Imaging

The Incucyte S3 is an automated microscope located within a standard cell culture incubator and is of particular interest for low resolution, long-term, live cell imaging. It allows the independent measurement and analysis of up to 6 plates or experiments at the same time. It is the method of choice for long-term observation of spheroid and organoid development.

More information about Incucyte long-term live cell imaging...

High Content Screening / Image Cytometry

Lighthouse has an Olympus Scan^R High Content Screening Station which allows automated image acquisition and data analysis of biological samples. Other terms for this field include image cytometry or automated microscopy.
More...

Nucleic Acid Analysis

Quantitative PCR

Lighthouse has two Roche LightCycler 480 (LC 480) machines for quantitative PCR analysis. Each LC 480 is a plate-based machine with both 384-well and 96-well blocks. The thermal blocks are fast-cycling, with typical run times slightly under one hour. The range of reaction volumes is 20 - 100 microliters for the 96-well and 5 - 20 microliters for the 384-well block.
More...

Droplet Digital PCR

In addition ti its Bio-Rad QX100 Droplet Digital PCR (ddPCR) system Lighthouse has just added a Stilla Naica Crystal Digital PCR system.
More...

Our Network

MIAP

Lighthouse is a member unit of the Microscopy and Image Analysis Platform (MIAP), an international network of microscopy and image analysis facilities from throughout the region, including Freiburg, Basel and Strasbourg.

Overview of MIAP Partner Units

Cytometry.de

Lighthouse is a part of cytometry.de, a national network of cytometrists and cytometry core facilities of the DGfZ / Deutsche Gesellschaft für Zytometrie.

RIsources

Lighthouse is listed within the RIsources Database of the DFG. The RIsources listing simplifies applying for funding for the use of Lighthouse services within DFG grant applications.

Lighthouse RIsources Page

Please note:

Our user fees cover only a small fraction of our total running costs, most of which in fact are covered by our home institutes (Klinik für Innere Medizin I, Centrum für Chronische Immundefizienz (CCI), Tumorzentrum Freiburg/DKTK (CCCF). The majority of our machines were financed with the help of outside sources such as the DFG, Land Baden-Württemberg, or BMBF. We therefore depend on proper acknowledgement in publications, grants and grant applications. Publications, posters or presentations that make use of services or data collected at the facility or by its staff should list these contributions accordingly. Thank you.

If you still have questions about the facility after reading through these pages, feel free to contact us at one of the numbers listed on the right side.

 

Universitätsklinikum Freiburg
Zentrum für Translationale Zellforschung (ZTZ)
Department of Medicine I
Tumorzentrum Freiburg - CCCF/DKTK
Center for Chronic Immune Deficiency - CCI

Contact | Information

 

FACS-Sorting Booking:

Telephone: 
Arias: 0761 270-63770
MoFlo: 0761 270-77680

Email:
facslab@uniklinik-freiburg.de

 

Online Reservations (for machines others than cell sorters):

Booking Calender (intranet only)
MoFlo Calender (read only)

 

Head of Facility:

Marie Follo, Ph.D.
Telephone: 0761 270-77697
Telefax: 0761 270-63780
marie.follo@uniklinik-freiburg.de

 

Address:

Universitätsklinikum Freiburg
Lighthouse Core Facility

Zentrum für Translationale Zellforschung (ZTZ)
Breisacher Straße 115
D-79106 Freiburg

www.core-facility.de