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Klinik für Innere Medizin IHämatologie, Onkologie und Stammzelltransplantation

Lighthouse Core Facility

 

 

The Lighthouse Core Facility was founded to give investigators access to a broad spectrum of fluorescence-based technologies useful in answering research questions in the areas of cell and molecular biology. For more information about what instruments and services are available at the facility and how you can take advantage of what we have to offer, click on the appropriate sections below.

                                   

 

FACS-Sorter MoFlo Astrios out of order currently

Unfortunately one of our cell sortes is not avalable these days. But we can offer to serve you on ARIA III and ARIA FUSION as well.

SONY Spectral Analyzer out of order

Unfortunately this machine is waiting for repair these days

IncuCyte S3 out of order

Unfortunately the IncuCyte needs a repair.

Attention, please!

Corona virus / SARS-CoV2 / COVID-19

Unfortunately, because of the increasing problems due to the coronavirus, we are not allowed to teach new users how to use our equipment. This is a temporary measure. At the current time we are postponing new introductions or "Einweisungen" until after Easter. After Easter the situation will be reevaluated.

Because new users naturally need the most intensive help or support, and we cannot guarantee we will be able to offer this as required as the situation is changing daily, putting a pause on new introductions is one of the steps we have decided to take.

We are trying to decrease the amount of foot traffic here in the facility as best as we can. We ask for your understanding in the current situation and we look forward to teaching you once things have settled down.

Due to the spread of the SARS-CoV2/coronavirus, access to the Lighthouse Core Facility is now being limited

All training sessions for new users have been suspended for the time being. You can email us (facslab@uniklinik-freiburg.de) about being placed on a waiting list for the next available training session, once we are able to offer them again.

Experienced users are allowed to continue to use our equipment but only when following these rules and restrictions: 

  • All access is prohibited to researchers who have not already been registered as a user in our sytem. 
  • The facility is accessible only to those without symptoms of illness.
  • Users must wash or disinfect their hands upon entering and leaving the facility.
  • Gloves must be worn by all users of our equpiment.
  • Either a personal lab coat or a fresh lab coat from the hall must be used. No sharing of lab coats is allowed.
  • A minimum distance of 2 meters between users must be maintained at all times.
  • Each machine may be used by only one user at a time.

Furthermore, additional restrictions for microscope users apply:

  • Only 1 person per imaging room is allowed!
  • Microscope users must wear laboratory safety goggles or glasses when using our equipment. We recommend that you also use a face mask.
  • Disinfect all areas you will touch (for example, keyboard, mouse, focus knobs, joysticks, oculars), before and after using the system with the available disinfection liquid available near the microscope.
  • In addition we have made cling film available for you at the facility main entrance which you can use to cover the oculars and surrounding areas.

Disinfection liquid is also located in every bay of the main lab and we urge you to use it to disinfect the computer keyboard, mouse and equipment controls before using the equipment.

 

 

Position Available - Lighthouse Core Facility

Sorter Operator

Ready to join an enthusiastic team with the opportunity to learn new skills and expand your horizons?

Lighthouse currently has an open position for a sort operator.

For more information and job application please follow the link.

 

Flow Cytometry

Cell Sorting

Available on Becton Dickinson's FACSAria III and FACSAria Fusion and Beckman Coulter's MoFlo Astrios EQ.
More...

Laser beams in a Fortessa

FACS Analysis

Available on Becton Dickinson's LSR Fortessas, Canto, Beckman Coulter's Gallios & Navios, and Sony's Spectral Analyser SP6800.
More...

Microscopy
Green, red and blue fluorescence in a cell

Confocal Microscopy

For confocal microscopy we have microscopes from Zeiss (LSM 880 with AiryScan) and LSM 710 and Leica's TCS SP2 AOBS available.
More...

Long-Term Live Cell Imaging

The Incucyte S3 is an automated microscope located within a standard cell culture incubator and is of particular interest for low resolution, long-term, live cell imaging. It allows the independent measurement and analysis of up to 6 plates or experiments at the same time. It is the method of choice for long-term observation of spheroid and organoid development.

More information about Incucyte long-term live cell imaging...

High Content Screening / Image Cytometry

Lighthouse has an Olympus Scan^R High Content Screening Station which allows automated image acquisition and data analysis of biological samples. Other terms for this field include image cytometry or automated microscopy.
More...

Nucleic Acid Analysis

Quantitative PCR

Lighthouse has two Roche LightCycler 480 (LC 480) machines for quantitative PCR analysis. Each LC 480 is a plate-based machine with both 384-well and 96-well blocks. The thermal blocks are fast-cycling, with typical run times slightly under one hour. The range of reaction volumes is 20 - 100 microliters for the 96-well and 5 - 20 microliters for the 384-well block.
More...

Droplet Digital PCR

In addition ti its Bio-Rad QX100 Droplet Digital PCR (ddPCR) system Lighthouse has just added a Stilla Naica Crystal Digital PCR system.
More...

Our Network

MIAP

Lighthouse is a member unit of the Microscopy and Image Analysis Platform (MIAP), an international network of microscopy and image analysis facilities from throughout the region, including Freiburg, Basel and Strasbourg.

Overview of MIAP Partner Units

Cytometry.de

Lighthouse is a part of cytometry.de, a national network of cytometrists and cytometry core facilities of the DGfZ / Deutsche Gesellschaft für Zytometrie.

RIsources

Lighthouse is listed within the RIsources Database of the DFG. The RIsources listing simplifies applying for funding for the use of Lighthouse services within DFG grant applications.

Lighthouse RIsources Page

Please note:

Our user fees cover only a small fraction of our total running costs, most of which in fact are covered by our home institutes (Klinik für Innere Medizin I, Centrum für Chronische Immundefizienz (CCI), Tumorzentrum Freiburg/DKTK (CCCF). The majority of our machines were financed with the help of outside sources such as the DFG, Land Baden-Württemberg, or BMBF. We therefore depend on proper acknowledgement in publications, grants and grant applications. Publications, posters or presentations that make use of services or data collected at the facility or by its staff should list these contributions accordingly. Thank you.

If you still have questions about the facility after reading through these pages, feel free to contact us at one of the numbers listed on the right side.

 

Universitätsklinikum Freiburg
Zentrum für Translationale Zellforschung (ZTZ)
Department of Medicine I
Tumorzentrum Freiburg - CCCF/DKTK
Center for Chronic Immune Deficiency - CCI

Contact | Information

 

FACS-Sorting Booking:

Email:
Facslab-email

 

Online Reservations (for machines others than cell sorters):

Booking Calender (intranet only)
MoFlo Calender (read only)

 

Head of Facility:

Marie Follo, Ph.D.
Telephone: 0761 270-77697
Telefax: 0761 270-63780
Email: Marie Follo

 

Address:

Universitätsklinikum Freiburg
Lighthouse Core Facility

Zentrum für Translationale Zellforschung (ZTZ)
Breisacher Straße 115
D-79106 Freiburg

www.core-facility.de