Zu den Inhalten springen

Klinik für Innere Medizin IHämatologie, Onkologie und Stammzelltransplantation

Lighthouse Core Facility

The Lighthouse Core Facility was founded to give investigators access to a broad spectrum of fluorescence-based technologies useful in answering research questions in the areas of cell and molecular biology. For more information about what instruments and services are available at the facility and how you can take advantage of what we have to offer, click on the appropriate sections below.

Universitätsklinikum Freiburg
Zentrum für Translationale Zellforschung (ZTZ)
Department of Medicine I
Tumorzentrum Freiburg - CCCF/DKTK
Center for Chronic Immune Deficiency - CCI

FACS-Sorting Booking:

Online Booking Calendar (registration required)
Facslab-email

Head of Facility:

Marie Follo, Ph.D.
Telephone: 0761 270-77697
Telefax: 0761 270-63780
Email: Marie Follo

Address:

Universitätsklinikum Freiburg
Lighthouse Core Facility

Zentrum für Translationale Zellforschung (ZTZ)
Breisacher Straße 115
D-79106 Freiburg

www.core-facility.de

COVID-19 / SARS-CoV2 / Corona Information

Lighthouse Core Facility Rules & Measures

Due to the spread of the SARS-CoV2/coronavirus, access to the Lighthouse Core Facility is currently limited.

Training sessions for new users are limited due to the current situation.

You can email us (facslab(ät)uniklinik-freiburg.de) about signing up for the next available training session.

Experienced users are allowed to continue to use our equipment but only when following these rules and restrictions (see PDF for download):

 

Fortessa Trainings

Content of the Basic Fortessa Introduction Course

  • Principle & construction of a flow cytometer
  • Startup operations, operation and shutdown procedures
  • Replacement of sheath and waste canisters
  • Sample preparation
  • Sample measurement
  • Spectral overlap compensation
  • Operating FACSDiva software
  • Troubleshooting
  • Operating booking system and input screen (for billing)
  • Optional: More detailed informations about FACSDiva & flow cytometry

Since June 2020 we offer the newly designed training courses for the FACS Fortessas. 
The regulations for keeping the minimum distance make it impossible to keep the previous training form. Therefore, we are switching to largely contact-free online training. 
If you would like to use our Fortessa flow cytometers (or the Canto II) and have not yet received instruction on these instruments from us, please send us an email to fortessa(ät)core-facility.de. You will then receive an email from us with all details and information.

Among them are: 

  • several links to instructional videos
  • an on-site appointment for questions and the short written test

You will then have about 1 week time to watch the videos and fill out forms if necessary.

At the individual on-site appointment (duration about 30 mins), the formalities will then be completed and, if possible, the authorization to use the equipment will be granted.

The next dates for the on-site appointments and final exams are planned for:

  • 02.12.2020
  • 17.12.2020
  • 12.01.2021
  • 27.01.2021
  • 11.02.2021
  • 25.02.2021
  • 09.03.2021
  • 23.03.2021
  • 07.04.2021
  • to be continued

SONY Spectral Analyzer

We offer basic user introductions for the Sony FACS Analyzer in the Lighthouse Core Facility.

The event is limited to a max. no. of 2 participants. The introduction can be in German or English. If you are interested please email us (sony(ät)core-facility.de and date of the training you would like to book) for a binding reservation.

Where: Lighthouse Core Facility, ZTZ, ground floor, room 009, bay 3 at the Sony Analyzer

When: All possible dates and times are here...

Visiting any introduction is free of charge and required before using the Sony Analyzer at the Lighthouse Core Facility.

Please bring pen and paper for your notes in any case and your own lab coat if you are based at ZTZ.

Note: The number of participants is limited and the registration is binding after confirmation by us. If you are unable to attend, please let us know as soon as possible. So there is still a chance for substitutes.

More information about the course.

MIAP

Lighthouse is a member unit of the Microscopy and Image Analysis Platform (MIAP), an international network of microscopy and image analysis facilities from throughout the region, including Freiburg, Basel and Strasbourg.

Overview of MIAP Partner Units

Cytometry.de

Lighthouse is a part of cytometry.de, a national network of cytometrists and cytometry core facilities of the DGfZ / Deutsche Gesellschaft für Zytometrie.

RIsources

Lighthouse is listed within the RIsources Database of the DFG. The RIsources listing simplifies applying for funding for the use of Lighthouse services within DFG grant applications.

Lighthouse RIsources Page

Please note:

Our user fees cover only a small fraction of our total running costs, most of which in fact are covered by our home institutes (Klinik für Innere Medizin I, Centrum für Chronische Immundefizienz (CCI), Tumorzentrum Freiburg/DKTK (CCCF). The majority of our machines were financed with the help of outside sources such as the DFG, Land Baden-Württemberg, or BMBF. We therefore depend on proper acknowledgement in publications, grants and grant applications. Publications, posters or presentations that make use of services or data collected at the facility or by its staff should list these contributions accordingly. Thank you.

If you still have questions about the facility after reading through these pages, feel free to contact us at one of the numbers listed above.