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Klinik für Innere Medizin IHämatologie, Onkologie und Stammzelltransplantation

Lighthouse Core Facility

 

 

 

The Lighthouse Core Facility was founded to give investigators access to a broad spectrum of fluorescence-based technologies useful in answering research questions in the areas of cell and molecular biology. For more information about what instruments and services are available at the facility and how you can take advantage of what we have to offer, click on the appropriate sections below.

Lighthouse Core Facility Logo

Spectral Analyzer from SONY

Our latest flow cytometer is a SONY SP6800 ZE Spectral Analyzer. It has a novel spectral detector, allowing the unmixing (separation) and analysis of multiple, even spectrally overlapping fluorochromes, simultaneously.

SONY Training on 06.11.2018

A basic user introduction for the Sony FACS Analyzer will take place in the Lighthouse Core Facility.

The event is limited to a max. no. of 5 participants. The introduction will be in English. If you are interested please email us for a binding reservation.

Where: Lighthouse Core Facility, ZTZ, ground floor, room 009, bay 3 at the Sony Analyzer

When: 06.Nov.2018 (from 9.00 AM until 11.30 AM)

Visiting any introduction is free of charge and required before using the Sony Analyzer at the Lighthouse Core Facility.

Please bring pen and paper for your notes in any case and your own lab coat if you are based at ZTZ.

FACS Fortessa Introduction 27.11.2018

The basic user introduction on FACS Analyser "Fortessa" takes place in the Core Facility. The event is limited to a maximum number of 5 participants. The introduction could be in English on request. If you are interested please contact us by email for a binding reservation. Please let us know your name, lab, phone number and the date of the training. We will inform you.

Where: Lighthouse Core Facility, ZTZ, ground floor, room 009, bay 2 at Fortessa "Frida"

When: 27. Nov. 2018 from 9.00 AM to 11.30 AM

Visiting of the introduction is free of charge and required for using our Fortessas at Lighthouse Core Facility. Please bring pen and paper for your notes in any case and your own lab coat if you are based at ZTZ.

FACS Fortessa Introduction 10.12.2018

The basic user introduction on FACS Analyser "Fortessa" takes place in the Core Facility. The event is limited to a maximum number of 5 participants. The introduction could be in English on request. If you are interested please contact us by email for a binding reservation. Please let us know your name, lab, phone number and the date of the training. We will inform you.

Where: Lighthouse Core Facility, ZTZ, ground floor, room 009, bay 2 at Fortessa "Frida"

When: 10. Dec. 2018 from 9.00 AM to 11.30 AM

Visiting of the introduction is free of charge and required for using our Fortessas at Lighthouse Core Facility. Please bring pen and paper for your notes in any case and your own lab coat if you are based at ZTZ.

SONY Training 11.12.18

A basic user introduction for the Sony FACS Analyzer will take place in the Lighthouse Core Facility.

The event is limited to a max. no. of 5 participants. The introduction will be in English. If you are interested please email us for a binding reservation.

Where: Lighthouse Core Facility, ZTZ, ground floor, room 009, bay 3 at the Sony Analyzer

When:  11.Dec.2018 (from 9.00 AM until 11.30 AM)

Visiting any introduction is free of charge and required before using the Sony Analyzer at the Lighthouse Core Facility.

Please bring pen and paper for your notes in any case and your own lab coat if you are based at ZTZ.

FACS Fortessa Introduction - 09.01.2019

The basic user introduction on FACS Analyser "Fortessa" takes place in the Core Facility. The event is limited to a maximum number of 5 participants. The introduction could be in English on request. If you are interested please contact us by email for a binding reservation. Please let us know your name, lab, phone number and the date of the training. We will inform you.

Where: Lighthouse Core Facility, ZTZ, ground floor, room 009, bay 2 at Fortessa "Frida"

When: 9. January 2019 from 9.00 AM to 11.30 AM

Visiting of the introduction is free of charge and required for using our Fortessas at Lighthouse Core Facility. Please bring pen and paper for your notes in any case and your own lab coat if you are based at ZTZ.

SONY Training 11.01.19

A basic user introduction for the Sony FACS Analyzer will take place in the Lighthouse Core Facility.

The event is limited to a max. no. of 5 participants. The introduction will be in English. If you are interested please email us for a binding reservation.

Where: Lighthouse Core Facility, ZTZ, ground floor, room 009, bay 3 at the Sony Analyzer

When:  11.01.2019 (from 9.00 AM until 11.30 AM)

Visiting any introduction is free of charge and required before using the Sony Analyzer at the Lighthouse Core Facility.

Please bring pen and paper for your notes in any case and your own lab coat if you are based at ZTZ.

FACS Fortessa Introduction - 21.01.19

The basic user introduction on FACS Analyser "Fortessa" takes place in the Core Facility. The event is limited to a maximum number of 5 participants. The introduction could be in English on request. If you are interested please contact us by email for a binding reservation. Please let us know your name, lab, phone number and the date of the training. We will inform you.

Where: Lighthouse Core Facility, ZTZ, ground floor, room 009, bay 2 at Fortessa "Frida"

When: 21. January 2019 from 9.00 AM to 11.30 AM

Visiting of the introduction is free of charge and required for using our Fortessas at Lighthouse Core Facility. Please bring pen and paper for your notes in any case and your own lab coat if you are based at ZTZ.

FACS Fortessa Introduction - 06.02.19

The basic user introduction on FACS Analyser "Fortessa" takes place in the Core Facility. The event is limited to a maximum number of 5 participants. The introduction could be in English on request. If you are interested please contact us by email for a binding reservation. Please let us know your name, lab, phone number and the date of the training. We will inform you.

Where: Lighthouse Core Facility, ZTZ, ground floor, room 009, bay 2 at Fortessa "Frida"

When: 06. Feb. 2019 from 9.00 AM to 11.30 AM

Visiting of the introduction is free of charge and required for using our Fortessas at Lighthouse Core Facility. Please bring pen and paper for your notes in any case and your own lab coat if you are based at ZTZ.

SONY Training 08.02.19

A basic user introduction for the Sony FACS Analyzer will take place in the Lighthouse Core Facility.

The event is limited to a max. no. of 5 participants. The introduction will be in English. If you are interested please email us for a binding reservation.

Where: Lighthouse Core Facility, ZTZ, ground floor, room 009, bay 3 at the Sony Analyzer

When:  08.02.2019 (from 9.00 AM until 11.30 AM)

Visiting any introduction is free of charge and required before using the Sony Analyzer at the Lighthouse Core Facility.

Please bring pen and paper for your notes in any case and your own lab coat if you are based at ZTZ.

FACS Fortessa Introduction 27.02.19

The basic user introduction on FACS Analyser "Fortessa" takes place in the Core Facility. The event is limited to a maximum number of 5 participants. The introduction could be in English on request. If you are interested please contact us by email for a binding reservation. Please let us know your name, lab, phone number and the date of the training. We will inform you.

Where: Lighthouse Core Facility, ZTZ, ground floor, room 009, bay 2 at Fortessa "Frida"

When: 27. Feb. 2019 from 9.00 AM to 11.30 PM

Visiting of the introduction is free of charge and required for using our Fortessas at Lighthouse Core Facility. Please bring pen and paper for your notes in any case and your own lab coat if you are based at ZTZ.

SONY Training 01.03.19

A basic user introduction for the Sony FACS Analyzer will take place in the Lighthouse Core Facility.

The event is limited to a max. no. of 5 participants. The introduction will be in English. If you are interested please email us for a binding reservation.

Where: Lighthouse Core Facility, ZTZ, ground floor, room 009, bay 3 at the Sony Analyzer

When:  01.03.2019 (from 9.00 AM until 11.30 AM)

Visiting any introduction is free of charge and required before using the Sony Analyzer at the Lighthouse Core Facility.

Please bring pen and paper for your notes in any case and your own lab coat if you are based at ZTZ.

Flow Cytometry

Cell Sorting

Available on Becton Dickinson's FACSAria III and FACSAria Fusion and Beckman Coulter's MoFlo Astrios EQ.
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Laser beams in a Fortessa

FACS Analysis

Available on Becton Dickinson's LSR Fortessas, Canto and Beckman Coulter's Gallios & Navios and Sony's Spectral Analyser SP6800.
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Microscopy
Green, red and blue fluorescence in a cell

Confocal Microscopy

For confocal microscopy we have microscopes from Zeiss (LSM 880 with AiryScan) and LSM 710 and Leica's TCS SP2 AOBS available.
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High Content Screening / Image Cytometry

Lighthouse has an Olympus Scan^R High Content Screening Station which allows automated image acquisition and data analysis of biological samples. Other terms for this field include image cytometry or automated microscopy.
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Nucleic Acid Analysis

Quantative PCR

Lighthouse has two Roche LightCycler 480 (LC 480) machines for quantitative PCR analysis. Each LC 480 is a plate-based machine with both 384-well and 96-well blocks. The thermal blocks are fast-cycling, with typical run times slightly under one hour. The range of reaction volumes is 20 - 100 microliters for the 96-well and 5 - 20 microliters for the 384-well block.
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Droplet Digital PCR

Lighthouse has a QX100 Droplet Digital PCR (ddPCR) system from Bio-Rad, which can be used in combination with TaqMan / Hydrolysis Probe-based assays. Currently, up to two colors can be used simultaneously (FAM and VIC/Hex). 
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Our Network

MIAP

Lighthouse is a member unit of the Microscopy and Image Analysis Platform (MIAP), an international network of microscopy and image analysis facilities from throughout the region, including Freiburg, Basel and Strasbourg.

Overview of MIAP Partner Units

Cytometry.de

Lighthouse is a part of cytometry.de, a national network of cytometrists and cytometry core facilities of the DGfZ / Deutsche Gesellschaft für Zytometrie.

RIsources

Lighthouse is listed within the RIsources Database of the DFG. The RIsources listing simplifies applying for funding for the use of Lighthouse services within DFG grant applications.

Lighthouse RIsources Page

Please note:

Our user fees cover only a small fraction of our total running costs, most of which in fact are covered by our home institutes (Klinik für Innere Medizin I, Centrum für Chronische Immundefizienz (CCI), Tumorzentrum Freiburg/DKTK (CCCF). The majority of our machines were financed with the help of outside sources such as the DFG, Land Baden-Württemberg, or BMBF. We therefore depend on proper acknowledgement in publications, grants and grant applications. Publications, posters or presentations that make use of services or data collected at the facility or by its staff should list these contributions accordingly. Thank you.

If you still have questions about the facility after reading through these pages, feel free to contact us at one of the numbers listed on the right side.

 

Universitätsklinikum Freiburg
Zentrum für Translationale Zellforschung (ZTZ)
Department of Medicine I
Tumorzentrum Freiburg - CCCF/DKTK
Center for Chronic Immune Deficiency - CCI

Contact | Information

 

FACS-Sorting Booking:

Telephone: 
Arias: 0761 270-63770
MoFlo: 0761 270-77680

Email:
facslab@uniklinik-freiburg.de

 

Online Reservations (for machines others than cell sorters):

Booking Calender (intranet only)
MoFlo Calender (read only)

 

Head of Facility:

Marie Follo, Ph.D.
Telephone: 0761 270-77697
Telefax: 0761 270-63780
marie.follo@uniklinik-freiburg.de

 

Address:

Universitätsklinikum Freiburg
Lighthouse Core Facility

Zentrum für Translationale Zellforschung (ZTZ)
Breisacher Straße 115
D-79106 Freiburg

www.core-facility.de