Microscopy and Image Analysis
Classical, fluorescence and confocal
Complex Microscope Systems
Lighthouse has recently installed an
Akoya Phenocycler-Fusion (CODEX) (ZTZ)
which allows the analysis of up to 100+ markers on a single slide.
Confocal and Lightsheet Microscopy
ZTZ: Zeiss LSM 880 with AiryScan
ZTZ: Zeiss LSM 710
IMTATE: Zeiss LSM 980 NLO (multiphoton) with AiryScan
IMTATE: Zeiss CellDiscoverer 7 with LSM 900 AiryScan Scanhead
IMTATE: Zeiss Lightsheet 7
High Content Screening (HCS)/ Automated Microscopy
Microscopes for Image Cytometry/Automated Microscopy/HCS:
ZTZ: Olympus scanR High Content Screening Station
IMITATE: Zeiss CellDiscoverer 7 with LSM 900 AiryScan
Slide Scanning Zeiss AxioScan 7 (IMITATE)
High capacity slide scanner for fluorescence or brightfield (IHC) samples. (Located in the IMITATE building)
Long-Term Live Cell Imaging Sartorius Incucyte S3
Lighthouse has a Sartorius Incucyte S3 system, which is an automated microscope contained within an incubator. It allows the independent imaging and analysis of up to 6 plates or experiments simultaneously.
Additional Widefield Systems
Zeiss AxioImager M2m (ZTZ)
Upright widefield microscope for immunohistochemistry and fluorescence microscopy
Configuration
Zeiss AxioObserver I / Apotome (ZTZ) Zeiss AxioOvserver II / Apotome (ZTZ)
Inverted fluorescence microscopes with on-stage climate chamber or heated stage, and motorized stage
Configurations
Zeiss Discovery.V8 (ZTZ)
Stereomicroscope. Brightfield and fluorescence (GFP/RFP).
Image Analysis
Bitplane Imaris - 3D reconstruction and analysis
AutoQuant / AutoDeblur - Deconvolution and 3D reconstruction
Metamorph - Image Analysis
Zeiss Zen Blue / Black
Olympus Scan^R analysis
Olympus Xcellence
Cell Profiler
ImageJ / FiJi
Biosafety Information
Live cell imaging of samples of biosafety level S2 (Gentechnikgesetz/ Infektionsschutzgesetz) requires that you first fill out a Biosafety Form. It is not necessary to fill out the checklist for every appointment. However, it should be filled out for each project, including experiments involving different cell types, treatments or sample origin.