Microscopy and Image Analysis
Classical, fluorescence and confocal
Confocal Microscopy
Lighthouse has three spectral confocal microscopes available for use:
- Zeiss LSM 880 with AiryScan
- Zeiss LSM 710
- Leica TCS SP2 AOBS
High Content Screening (HCS) / Image Cytometry
In the areas of HCS / Image Cytometry / Automated Microscopy Lighthouse has an:
- Olympus Scan^R High Content Screening Station
More information about the Scan^R system
Long-Term Live Cell Imaging
Long-Term Live Cell Imaging
The Incucyte S3 is an automated microscope located within a standard cell culture incubator and is of particular interest for low resolution, long-term, live cell imaging. It allows the independent measurement and analysis of up to 6 plates or experiments at the same time. It is the method of choice for long-term observation of spheroid and organoid development.
More information about Incucyte long-term live cell imaging...
Other Systems
Lighthouse also has several smaller microscope systems:
| Zeiss AxioImager M2m | Upright Microscope for immunohistochemistry and fluorescence Configuration |
| Zeiss AxioObserver.Z1 / Apotome | Inverted fluorescence microscope,with climate chamber and motorized stage Configuration |
| Zeiss Discovery.V8 | Stereomicroscope |
Image Analysis
Several workstations are available for offline analysis. Available software includes:
- AutoQuant / AutoDeblur - Deconvolution and 3D Reconstruction
- Metamorph - Image Analysis
- Zeiss Zen Blue / Black
- Olympus Scan^R analysis
- Olympus Xcellence
- tibco Spotfire
- Cell Profiler
- ImageJ / FiJi
Additional Links
Universitätsklinikum Freiburg
Zentrum für Translationale Zellforschung (ZTZ)
Department of Medicine I
Tumorzentrum Freiburg - CCCF/DKTK
Center for Chronic Immune Deficiency - CCI
FACS-Sorting Booking:
Email:
Facslab-email
Online Reservations (for machines others than cell sorters):
Booking Calender (intranet only)
MoFlo Calender (read only)
Head of Facility:
Marie Follo, Ph.D.
Telephone: 0761 270-77697
Telefax: 0761 270-63780
Email: Marie Follo
Address:
Universitätsklinikum Freiburg
Lighthouse Core Facility
Zentrum für Translationale Zellforschung (ZTZ)
Breisacher Straße 115
D-79106 Freiburg