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Klinik für Innere Medizin IHämatologie, Onkologie und Stammzelltransplantation

Microscopy and Image Analysis

Classical, fluorescence and confocal

Long-Term Live Cell Imaging

Long-Term Live Cell Imaging

The Incucyte S3 is an automated microscope located within a standard cell culture incubator and is of particular interest for low resolution, long-term, live cell imaging. It allows the independent measurement and analysis of up to 6 plates or experiments at the same time. It is the method of choice for long-term observation of spheroid and organoid development.

More information about Incucyte long-term live cell imaging...

Confocal Microscopy

Lighthouse has three spectral confocal microscopes available for use:

  • Zeiss LSM 880 with AiryScan
  • Zeiss LSM 710
  • Leica TCS SP2 AOBS

More information about the confocal microscopes

High Content Screening (HCS) / Image Cytometry

In the areas of  HCS / Image Cytometry / Automated Microscopy Lighthouse has an:

  • Olympus Scan^R High Content Screening Station

More information about the Scan^R system

 

Other Systems

Lighthouse also has several smaller microscope systems:

Zeiss AxioImager M2mUpright Microscope for immunohistochemistry and fluorescence 
Configuration
Zeiss AxioObserver.Z1 / ApotomeInverted fluorescence microscope,with climate chamber and motorized stage
Configuration
Zeiss Discovery.V8Stereomicroscope

Image Analysis

Several workstations are available for offline analysis. Available software includes:

  • AutoQuant / AutoDeblur - Deconvolution and 3D Reconstruction
  • Metamorph - Image Analysis
  • Zeiss Zen Blue / Black
  • Olympus Scan^R analysis
  • Olympus Xcellence
  • tibco Spotfire
  • Cell Profiler
  • ImageJ / Fiji

Additional Links

 

Universitätsklinikum Freiburg
Zentrum für Translationale Zellforschung (ZTZ)
Department of Medicine I
Tumorzentrum Freiburg - CCCF/DKTK
Center for Chronic Immune Deficiency - CCI

Contact | Information

 

FACS-Sorting Booking:

Telephone: 
Arias: 0761 270-63770
MoFlo: 0761 270-77680

Email:
facslab@uniklinik-freiburg.de

 

Online Reservations (for machines others than cell sorters):

Booking Calender (intranet only)
MoFlo Calender (read only)

 

Head of Facility:

Marie Follo, Ph.D.
Telephone: 0761 270-77697
Telefax: 0761 270-63780
marie.follo@uniklinik-freiburg.de

 

Address:

Universitätsklinikum Freiburg
Lighthouse Core Facility

Zentrum für Translationale Zellforschung (ZTZ)
Breisacher Straße 115
D-79106 Freiburg

www.core-facility.de