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Klinik für Innere Medizin IHämatologie, Onkologie und Stammzelltransplantation

Microscopy

Long-Term Live Cell Imaging

Sartorius Incucyte S3

The Incucyte S3 is an automated microscope located within a standard cell culture incubator and is of particular interest for low resolution, long-term, live cell imaging. It allows the independent measurement and analysis of up to 6 plates or experiments at the same time. It is the method of choice for long-term observation of spheroid and organoid development. Available objectives include 4x, 10x and 20x. These objectives are compatible with most standard cell culture plate formats and plates of to 96- or 384-wells are also supported. For the list of currently supported substrates please see (link for download). Channels available for imaging and analysis are standard brightfield/phase contrast, as well as two channels for fluorescence. Details about fluorescence can be seen below:

Color Excitation Emission Possible Fluorochromes
Green fluorescence 440-480 nm 504-544 nm GFP, YFP, CellTracker Green, CFSE, FITC
Red fluorescence 565-605 nm 625-705 nm mRFP, mKate2, mCherry, CellTracker Red, Alexa Fluor 594

Note: The red fluorescence channel is suboptimal for the detection of fluorescence proteins such as td tomato and turbo RFP.

 

 

Plates and Flasks fitting into IncuCyte

The list for download contains the vessels which work with the IncuCyte.

Download

 

Universitätsklinikum Freiburg
Zentrum für Translationale Zellforschung (ZTZ)
Department of Medicine I
Tumorzentrum Freiburg - CCCF/DKTK
Center for Chronic Immune Deficiency - CCI

Contact | Information

 

FACS-Sorting Booking:

Telephone: 
Arias: 0761 270-63770
MoFlo: 0761 270-77680

Email:
facslab@uniklinik-freiburg.de

 

Online Reservations (for machines others than cell sorters):

Booking Calender (intranet only)
MoFlo Calender (read only)

 

Head of Facility:

Marie Follo, Ph.D.
Telephone: 0761 270-77697
Telefax: 0761 270-63780
marie.follo@uniklinik-freiburg.de

 

Address:

Universitätsklinikum Freiburg
Lighthouse Core Facility

Zentrum für Translationale Zellforschung (ZTZ)
Breisacher Straße 115
D-79106 Freiburg

www.core-facility.de