Klinik für Innere Medizin IHämatologie, Onkologie und Stammzelltransplantation

Lighhouse currently operates three spectral confocal microscopes:

Zeiss LSM 880 with AiryScan

Zeiss LSM 710

Leica TCS SP2 AOBS

Confocal microscopes can take images in which only the focal plane of a sample is visible. This results in an improved signal to noise ratio, as well as an increase in effective resolution relative to those made with a standard fluorescence microscope. Confocal microscopy is of potential interest in a wide range of experiments, for example those involving thick specimens, intracellular studies, and colocalization, as well as in studies where the three-dimensional structure of the sample is important. 

All of the systems are spectral confocal microscopes, and the lasers available on the different systems are listed below. 

Both Zeiss microscopes are equipped with climate control chambers for live cell imaging/timelapse measurements. The LSM 880 with AiryScan is a super resolution microscope which is able to take images of specimens beyond the diffraction limit.

Only those investigators who have been previously trained to use the microscopes by the Core Facility will be allowed to use them without an operator. For information about reserving time on the instruments, or about labeling strategies for your experiments, just contact the facility.

Lighthouse also has separate computer workstations with deconvolution, 3D reconstruction and image analysis programs which can be used to help analyze images and Z-stacks taken with the microscopes. For  freeware to help you with examination of  your images please see our confocal links of interest page below.