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Klinik für Innere Medizin IHämatologie, Onkologie und Stammzelltransplantation

Microscopy

Olympus Scan^R

Lighthouse has an Olympus Scan^R High Content Screening Station which allows automated image acquisition and data analysis of biological samples. Other terms for this field include image cytometry or automated microscopy.

The Scan^R is able to process large numbers of samples in an objective manner, enabling quantitative analysis across cells, wells, and samples. Although the total throughput is not as high as that of a flow cytometer it is still much higher than that of a typical microscope, allowing entire cell populations to be examined. Unlike flow cytometers, cells can be examined in situ, allowing morphological information from cells and tissue sections to be collected and used for analysis. However, the images acquired can be analyzed through the use of histograms, dot plots and even population gating as in flow cytometry.

Sample Types

Sample types which can be investigated with the Scan^R include both adherent and non-adherent cells and tissue sections. A climate-controlled chamber is available for living cells and time lapse experiments. 

Sample Templates 

The Scan^R is very flexible with regard to what templates can be used for the samples. However, plates and dishes with optical bottoms are recommended for the best quality images and lowest background.

  • Microtiter plates (96- or 384-well)
  • Chambered coverslips
  • Standard slides
  • Petri dishes
  • Standard tissue culture plates

Excitation/Emission 

The Scan^R is able to use a broad range of fluorochromes. Standard filter sets are listed below. 

ExcitationEmission Examples of Dyes or Proteins which Could Be Used
350 nm
455 nmDAPI,  Hoechst
458 nm477 nmECFP, Sytox Blue, BOPRO-1
488 nm
508 nm
EGFP, Alexa 488, FITC, CY2, YOYO-1, DiOC6(3)
514 nm 527 nmEYFP, TOTO-1, Oregon Green 514, Rhod-123, Bodipy-FL
546 nm 570 nmAlexa 546, Alexa 555, TRITC (Rhodamine), CY3, Propidium Iodide, dsRed, RFP, PE
594 nm
615 nm
Texas Red, Alexa 594, CY 3.5
633 nm
670 nm
Alexa 633, CY5, APC, TOTO-3, DRAQ-5
710 nm  775 nmCY7, Alexa 750

Additional filter sets are also available (i.e. Indo-1 for Calcium flux, and CY5.5 for some near-infrared Fluorescent Proteins). Although fluorescence is the first choice for quantitation, it is also possible to examine/quantify standard histologically stained/IHC specimens (brightfield) using the Scan^R.

If you have any questions about possible labelling strategies, we would be happy to help you. 

Access

Only those investigators who have been previously trained to use the Scan^R Screening Station are allowed to use it without an operator. For information about reserving time on the instrument, contact one of us.

Further Information

 

Universitätsklinikum Freiburg
Zentrum für Translationale Zellforschung (ZTZ)
Department of Medicine I
Tumorzentrum Freiburg - CCCF/DKTK
Center for Chronic Immune Deficiency - CCI

Contact | Information

 

FACS-Sorting Booking:

Telephone: 
Arias: 0761 270-63770
MoFlo: 0761 270-77680

Email:
facslab@uniklinik-freiburg.de

 

Online Reservations (for machines others than cell sorters):

Booking Calender (intranet only)
MoFlo Calender (read only)

 

Head of Facility:

Marie Follo, Ph.D.
Telephone: 0761 270-77697
Telefax: 0761 270-63780
marie.follo@uniklinik-freiburg.de

 

Address:

Universitätsklinikum Freiburg
Lighthouse Core Facility

Zentrum für Translationale Zellforschung (ZTZ)
Breisacher Straße 115
D-79106 Freiburg

www.core-facility.de