Zu den Inhalten springen

Cancer ResearchDepartment of Thoracic Surgery

Division of Cancer Research

Department of Thoracic Surgery

High-Impact Publications

Publications with Impact Factor 2019 >=10

S Maheswaran*, LV Sequist*, S Nagrath*, L Ulkus, B Brannigan, C Collura, E Inserra, S Diederichs, AJ Iafrate, DW Bell, S Digumarthy, A Muzikansky, D Irimia, J Settleman, RG Tompkins, TJ Lynch, M Toner, DA Haber: "Detecting Epidermal Growth Factor Receptor Mutations in Circulating Tumor Cells from Patients with Non-Small Cell Lung Cancer"
New England Journal of Medicine (2008) 359: 366-377
IF: 75

A Roth, S Diederichs: "RAP and ChIRP about X inactivation" 
Nature (2015) 521: 170-171
IF: 43

I Surolia*, SP Pirnie*, V Chellappa*, KN Taylor*, A Cariappa*, J Moya, H Liu, DW Bell, D Driscoll, S Diederichs, K Haider, I Netravali, S Le, R Elia, E Dow, A Lee, J Freudenberg, PL De Jager, Y Chretien, A Varki, ME MacDonald, T Gillis, TW Behrens, D Bloch, D Collier, J Korzenik, DK Podolsky, D Hafler, M Murali, B Sands, JH Stone, PK Gregersen, S Pillai: "Functionally defective germline variants of sialic acid acetylesterase in autoimmunity"
Nature (2010) 466: 243-247
IF: 43

S Diederichs, DA Haber: "Dual Role for Argonautes in microRNA Processing and post-transcriptional Regulation of microRNA Expression"
Cell (2007) 131: 1097-1108
IF: 39

M Klingenberg, A Matsuda, S Diederichs*, T Patel*: "Non-coding RNA in hepatocellular carcinoma: Mechanisms, biomarkers and therapeutic targets"
Journal of Hepatology (2017) 67: 603-618
IF: 21

S Dhamija*, CM Yang*, J Seiler, K Myacheva, M Caudron-Herger, A Wieland, M Abdelkarim, Y Sharma, M Riester, M Groß, J Maurer, S Diederichs: "A Pan-Cancer Analysis reveals Nonstop Extension Mutations causing SMAD4 Tumor Suppressor Degradation"
Nature Cell Biology (2020) epub
IF: 20

J Winter*, S Jung*, S Keller, RI Gregory, S Diederichs: "Many roads to maturity: microRNA biogenesis pathways and their regulation"
Nature Cell Biology (2009) 11: 228-234
IF: 20

H Sültmann, S Diederichs: "Long non-coding RNA: LNCs to cancer"
European Urology (2014) 65: 1152-1153
IF: 18

C Müller-Tidow, W Wang, GE Idos, S Diederichs, R Yang, C Readhead, WE Berdel, H Serve, M Saville, R Watson, HP Koeffler: "Cyclin A1 directly interacts with B-myb and Cyclin A1/cdk2 phosphorylate B-myb at functionally important serine and threonine residues: tissue-specific regulation of B-myb function"
Blood (2001) 97: 2091-2097
IF: 18

C Müller, R Yang, GE Idos, N Tidow, S Diederichs, OM Koch, W Verbeek, TP Bender, HP Koeffler: "c-myb Transactivates the Human Cyclin A1 Promoter and Induces Cyclin A1 Gene Expression"
Blood (1999) 94: 4255-4262
IF: 18

M Caudron-Herger
, SF Rusin, ME Adamo, J Seiler, V Schmid, E Barreau, AN Kettenbach, S Diederichs: "R-DeeP: Proteome-wide and Quantitative Identification of RNA-dependent Proteins by Density Gradient Ultracentrifugation"
Molecular Cell (2019) 75: 184-199
IF: 16

M Klingenberg, M Groß, A Goyal, M Polycarpou-Schwarz, T Miersch, AS Ernst, J Leupold, N Patil, U Warnken, H Allgayer, T Longerich, P Schirmacher, M Boutros, S Diederichs: "The lncRNA CASC9 and RNA binding protein HNRNPL form a complex and co-regulate genes linked to AKT signaling"
Hepatology (2018) 68: 1817-1832
IF: 15

T Gutschner*, M Hämmerle*, N Pazaitis*, N Bley, E Fiskin, H Uckelmann, A Heim, M Groß, N Hofmann, R Geffers, B Skawran, T Longerich, K Breuhahn, P Schirmacher, B Mühleck, S Hüttelmaier*, S Diederichs*: "The Insulin-like Growth Factor 2 mRNA-Binding Protein 1 (IGF2BP1) is an important pro-tumorigenic factor in hepatocellular carcinoma"
Hepatology (2014) 59: 1900-1911
IF: 15

L Quagliata, MS Matter, S Piscuoglio, L Arabi, C Ruiz, A Procino, M Kovac, F Moretti, Z Makowska, T Boldanova, JB Andersen, M Hämmerle, L Tornillo, MH Heim, S Diederichs, C Cillo*, LM Terracciano*: "IncRNA HOTTIP / HOXA13 expression is associated with disease progression and predicts outcome in hepatocellular carcinoma patients"
Hepatology (2014) 59: 911-923
IF: 15

M Hämmerle*, T Gutschner*, H Uckelmann, S Ozgur, E Fiskin, M Groß, B Skawran, R Geffers, T Longerich, K Breuhahn, P Schirmacher, G Stoecklin, S Diederichs: "Post-transcriptional destabilization of the liver-specific long non-coding RNA HULC by the IGF2 mRNA-binding protein 1 (IGF2BP1)"
Hepatology (2013) 58: 1703-1712
IF: 15

F Haderk, R Schulz, M Iskar, LL Cid, T Worst, KV Willmund, A Schulz, U Warnken, J Seiler, A Benner, M Nessling, T Zenz, M Göbel, J Dürig, S Diederichs, J Paggetti, E Moussay, S Stilgenbauer, M Zapatka, P Lichter, M Seiffert: "Tumor-derived exosomes modulate PD-L1 expression in monocytes"
Science Immunology (2017) 2: eaah5509
IF: 13

M Gandhi, M Caudron-Herger, S Diederichs
:"RNA motifs and combinatorial
prediction of non-coding RNA interaction, stability and localization"
Nature Structural & Molecular Biology (2018) 25: 1070-1076
IF: 12

S Diederichs: "microTerminator: 'Hasta la vista, lncRNA !' "
Nature Structural & Molecular Biology (2015) 22: 279-281
IF: 12

M Gandhi, M Groß, JM Holler, SA Coggins, N Patil, JH Leupold, M Munschauer, M Schenone, CR Hartigan, H Allgayer, B Kim, S Diederichs: "The lncRNA lincNMR regulates nucleotide metabolism via a YBX1 - RRM2 axis in cancer"
Nature Communications (2020) 11: 3214
IF: 12

Y Sharma*, M Miladi*, S Dukare*, K Boulay*, M Caudron-Herger, M Groß, R Backofen, S Diederichs: "A pan-cancer analysis of synonymous mutations"
Nature Communications (2019) 10: 2569
IF: 12

D Nachmani, T Gutschner, A Reches, S Diederichs*, O Mandelboim*: "RNA binding proteins regulate the expression of the immune activating ligand MICB"
Nature Communications (2014) 5: 4186
IF: 12

M Miladi*, M Raden*, S Diederichs, R Backofen: "MutaRNA: analysis and visualisation of mutation-induced changes in RNA structure"
Nucleic Acids Research (2020) 37: epub
IF: 12

Q Sun*, V Tripathi*,#, JH Yoon#, DK Singh, Q Hao, KW Min, S Davila, RW Zealy, XL Li, M Polycarpou-Schwarz, E Lehrmann, Y Zhang, KG Becker, SM Freier, Y Zhu, S Diederichs, SG Prasanth, A Lal, M Gorospe, KV Prasanth#: "MIR100 host gene-encoded lncRNAs regulate cell cycle by modulating the interaction between HuR and its target mRNAs"
Nucleic Acids Research (2018) 46: 10405-10416
IF: 12

G Dumbović, J Biayna, J Banús, J Samuelsson, A Roth, S Diederichs, S Alonso, M Buschbeck, M Perucho*, SV Forcales*: "A novel long non-coding RNA from NBL2 pericentromeric macrosatellite forms a perinucleolar aggregate structure in colon cancer"
Nucleic Acids Research (2018) 46: 5504-5524
IF:12

T Mlambo, S Nitsch, M Hildenbeutel, M Romito, M Müller, C Bossen, S Diederichs, T Cornu, T Cathomen, C Mussolino: "Designer epigenome modifiers enable robust and sustained gene silencing in clinically relevant human cells"
Nucleic Acids Research (2018) 46: 4456-4468
IF: 12

R Soares, G Maglieri, T Gutschner, S Diederichs, A Lund, B Nielsen, K Holmstrøm: "Evaluation of fluorescence in situ hybridization techniques to study long non-coding RNA expression in cultured cells"
Nucleic Acids Research (2018) 46: e4
IF: 12 

A Goyal, E Fiskin, T Gutschner, M Polycarpou-Schwarz, M Groß, J Neugebauer, M Gandhi, M Caudron-Herger,
V Benes, S Diederichs: "A cautionary tale of sense-antisense gene pairs: Independent regulation despite inverse correlation of expression"
Nucleic Acids Research (2017) 45: 12496-12508
IF: 12

J Seiler, M Breinig, M Caudron-Herger, M Polycarpou-Schwarz, M Boutros, S Diederichs: "The lncRNA VELUCT strongly regulates viability of lung cancer cells despite its extremely low abundance"
Nucleic Acids Research (2017) 45: 5458-5469
IF: 12 

A Goyal, K Myacheva, M Groß, M Klingenberg, B Duran-Arque, S Diederichs: "Challenges of CRISPR / Cas9 applications for long non-coding RNA genes" 
Nucleic Acids Research (2017) 45: e12
IF: 12 

S Link*, S Grund*, S Diederichs: "Alternative splicing affects the subcellular localization of Drosha"
Nucleic Acids Research (2016) 44: 5330-5343
IF: 12 

J Haar, M Contrant, K Bernhardt, R Feederle, S Diederichs, S Pfeffer, HJ Delecluse: "The expression of a viral microRNA is regulated by clustering to allow optimal B cell transformation"
Nucleic Acids Research (2016) 44: 1326-1341
IF: 12

J Winter, S Link, D Witzigmann, C Hildenbrand, C Previti, S Diederichs: "Loop-miRs: Active microRNAs generated from single-stranded loop regions"
Nucleic Acids Research (2013) 41: 5503-5512
IF: 12

T Palm, K Hemmer, J Winter, I Fricke, K Tarbashevich, F Sadegi Shakib, I Rudolph, A Hillje, P De Lucca, L Bahnassawy, R Madel, T Viel, A de Siervi, A Jacobs, S Diederichs, J Schwamborn: "A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop"
Nucleic Acids Research (2013) 41: 3699-3712
IF: 12

T Gutschner, M Baas, S Diederichs: "Non-coding RNA gene silencing through genomic integration of RNA destabilizing elements using zinc finger nucleases"
Genome Research (2011) 21: 1944-1954
IF: 11

S Diederichs: "The four dimensions of non-coding RNA conservation"
Trends in Genetics (2014) 30: 121-123
IF: 11

M Caudron-Herger, E Wassmer, I Nasa, AS Schultz, J Seiler, AN Kettenbach, S Diederichs: "Identification, quantification and bioinformatic analysis of RNA-dependent proteins by RNase treatment and density gradient ultracentrifugation using R-DeeP"
Nature Protocols (2020) 15: 1338-1370
IF: 10

RM de Guia, AJ Rose, A Sommerfeld, O Seibert, D Strzoda, A Zota, Y Feuchter, A Krones-Herzig, T Sijmonsma, M Kirilov, C Sticht, N Gretz, G Dallinga-Thie, S Diederichs, N Klöting, M Blüher, M Berriel Diaz, S Herzig:"microRNA-379 couples glucocorticoid hormones to dysfunctional lipid homeostasis"
EMBO Journal (2015) 34: 344-360
IF: 11

G Stoecklin, S Diederichs: "tRNAs: New tricks from old dogs"
EMBO Journal (2014) 33: 1981-1983
IF: 10

Division of Cancer Research

Department of Thoracic Surgery

Center for Translational Cell Research (ZTZ)
Breisacher Str. 115
D-79106 Freiburg

 

Prof. Dr. Sven Diederichs

Division Head

Phone:
+49 761 270-77571
E-Mail:
lung-cancer-research
@uniklinik-freiburg.de
 

More than 70% of the human Genome (blue) is transcribed into RNA forming the Transcriptome (red), while only less than 2% of the genome are needed to produce all proteins in a human cell constituting the Proteome (yellow).

RNA Biology

A particular emphasis of our research lies on the Molecular Biology of RNA and its associated molecules.

Recent insights into RNA biology induced a paradigm shift towards the recognition of RNAs as functionally important molecules - beyond serving as messengers for protein-encoding genes. A large fraction of the human genome is transcribed into RNA (more than 70%), while only 2% are protein-encoding. Non-protein-coding RNAs execute important functions in the cell. Very short non-coding RNAs, the microRNAs, play important roles in gene regulation. The tumor-suppressive or oncogenic role of many microRNAs and their frequent deregulation in tumors allow a first glimpse of the striking role that non-coding RNAs could play in cancer. Novel long non-coding RNAs (ncRNA, lncRNA, lincRNA) fulfill important functions in epigenetic regulation, chromatin remodeling or splicing. Taken together, the human cell contains many more RNAs than previously anticipated and many of them might just await their discovery as functionally important molecules in cancer.

Our research focuses on long non-coding RNAs (lncRNAs) and their role in cancer. Based on expression profiling using microarrays as well as deep RNA sequencing of the whole transcriptome, we elucidate the cellular and molecular functions of differentially regulated ncRNAs in cancer using innovative techniques like the CRISPR/Cas9 system, in vivo RNA Affinity Purification and our own customized siRNA and CRISPR libraries targeting specifically lung cancer-associated lncRNAs. Our RNAi screens have uncovered numerous lncRNAs controlling several hallmarks of cancer including cancer cell viability, mitosis and Migration.

An emerging Topic of particular importance in our lab are RNA-Protein complexes, especially the new concept of RNA Dependence: R-DeeP (Mol Cell 2019).