Myeloid Cells
We are using a system where we can differentiate mouse haematopoietic progenitor cells into either neutrophils or macrophages. We have used this system to characterize spontaneous apoptosis (Haschka et al., 2015; Kirschnek et al., 2011) and immunodeficiencies (Gautam et al., 2013) in neutrophils and have looked into functions of neutrophils in more complex situations (with Prof. Zeiser, Haematology) (Hulsdunker et al., 2018; Schwab et al., 2014).
References
Gautam, S., Kirschnek, S., Gentle, I.E., Kopiniok, C., Henneke, P., Hacker, H., Malleret, L., Belaaouaj, A., and Hacker, G. (2013). Survival and differentiation defects contribute to neutropenia in glucose-6-phosphatase-beta (G6PC3) deficiency in a model of mouse neutrophil granulocyte differentiation. Cell death and differentiation 20, 1068-1079.
Haschka, M.D., Soratroi, C., Kirschnek, S., Hacker, G., Hilbe, R., Geley, S., Villunger, A., and Fava, L.L. (2015). The NOXA-MCL1-BIM axis defines lifespan on extended mitotic arrest. Nature communications 6, 6891.
Hulsdunker, J., Ottmuller, K.J., Neeff, H.P., Koyama, M., Gao, Z., Thomas, O.S., Follo, M., Al-Ahmad, A., Prinz, G., Duquesne, S., et al. (2018). Neutrophils provide cellular communication between ileum and mesenteric lymph nodes at graft-versus-host disease onset. Blood 131, 1858-1869.
Kirschnek, S., Vier, J., Gautam, S., Frankenberg, T., Rangelova, S., Eitz-Ferrer, P., Grespi, F., Ottina, E., Villunger, A., Hacker, H., et al. (2011). Molecular analysis of neutrophil spontaneous apoptosis reveals a strong role for the pro-apoptotic BH3-only protein Noxa. Cell death and differentiation 18, 1805-1814.
Schwab, L., Goroncy, L., Palaniyandi, S., Gautam, S., Triantafyllopoulou, A., Mocsai, A., Reichardt, W., Karlsson, F.J., Radhakrishnan, S.V., Hanke, K., et al. (2014). Neutrophil granulocytes recruited upon translocation of intestinal bacteria enhance graft-versus-host disease via tissue damage. Nature medicine 20, 648-654.