Zu den Inhalten springen

Institut für Medizinische Mikrobiologie und Hygiene

Prof. Dr. med. Georg Häcker

Telefon Labor+49 761 203-5362
Telefon Sekretariat+49 761 203-6532
Fax+49 761 203-6651
E-Mailgeorg.haecker@uniklinik-freiburg.de

CV

1983 Allgemeine Hochschulreife
1990 Medizinisches Staatsexamen
1991 Promotion zum Dr. med., Universität Ulm
1992Approbation als Arzt
1993-1995 Stipendiat der Deutschen Forschungsgemeinschaft am Walter und Eliza Hall Institute for Medical Research, Melbourne, Australien
1995Research Officer am Walter and Eliza Hall Institute
1996-1998Wissenschaftlicher Angestellter / Assistenzarzt, Inst. f. Med. Mikrobiologie der TUM
1998 Habilitation und Venia legendi für Medizinische Mikrobiologie und Immunologie, Fakultät für Medizin der TUM
1998-2000Privatdozent, Oberarzt am Inst. f. Med. Mikrobiologie der TUM
2000-2009C3-Professor / Extraordinarius, Oberarzt am Inst. f. Med. Mikrobiologie der TUM
2002Facharzt für Mikrobiologie und Infektionsepidemiologie, Bayerische Landesärztekammer
07/2009 Ärztlicher Direktor der Abteilung Mikrobiologie und Hygiene, Universitätsklinikum Freiburg

Who is who?

NamePositionTelefon E-Mail
Prof. Georg HäckerAG-Leiter203- 6531georg.haecker@uniklinik-freiburg.de
Dr. rer. nat. Arnim WeberWiss. Angestellter203- 5362arnim.weber@uniklinik-freiburg.de
Dr. rer. nat. Tina TzivelekidisWiss. Angestellte203- 5358tina.tzivelekidis@uniklinik-freiburg.de
Collins KontchouBiol. Doktorand203- 5358collins.kontchou@uniklinik-freiburg.de
Isabel MölterBiol. Doktorandin203- 5358isabel.moelter@uniklinik-freiburg.de
Michaela OhmerBiol. Doktorandin203- 5358michaela.ohmer@uniklinik-freiburg.de
Aris RoukounakisBiol. Doktorand203- 5358aris.roukounakis@uniklinik-freiburg.de
Dominik BrokatzkyBiol. Doktorandin203- 5358dominik.brokatzky@uniklinik-freiburg.de
Sophie KrügerMTLA203- 5363sophie.krueger@uniklinik-freiburg.de

Publikationsliste

  • Vier, J., Gerhard, M., Fürmann, C., Wagner, H and Häcker, G. (2004) Acceleration of CD95-Induced Caspase-8-Activation by Uncoupling of Mitochondrial Oxidative Phosphorylation. Mol. Immunol, 40: 661-670.
  • Fischer, S., Harlander, T., Vier, J. and Häcker, G. (2004) Protection against CD95-induced apoptosis by chlamydial infection at a mitochondrial step. Infect. Immun,72:1107-15.
  • Kirschnek, S., Scheffel, J., Heinzmann, U. and Häcker, G. (2004) Necrosis-like cell death induced by bacteria in mouse macrophages. Eur. J. Immunol. 34:1461-71.
  • Schliep, S., Decker, T., Schneller, F., Wagner, H. and Häcker, G. (2004) Functional Evaluation of the Role of Inhibitor of Apoptosis Proteins in Chronic Lymphocytic Leukemia. Exp. Hematol. 32:556-562.
  • Von Bubnoff, D., Bausinger, H., Matz, H., Koch, S., Häcker, G., Takikawa, O., Bieber, T., Hanau, D. and de la Salle, H. (2004) Human epidermal langerhans cells express the immunoregulatory enzyme indoleamine 2,3-dioxygenase. J. Invest. Dermatol. 123:298-304.
  • Ruckdeschel, K., Pfaffinger, G., Haase, R., Sing, A., Weighard, H., Häcker, G., Holzmann, H. and Heesemann, J. (2004) Signaling of apoptosis through toll-like receptors critically involves Toll/IL-1 receptor domain containing adapter inducing IFN- but not myeloid differentiation factor 88 in bacteria-infected murine macrophages. J. Immunol., 173:3320-3328.
  • Kreuz, S., Siegmund, D., Rumpf, J.J., Samel, D., Leverkus, M., Janssen, O., Häcker, G., Dittrich-Breiholz, O., Kracht, M., Scheurich, P. and Wajant, H. (2004) NFB activation by Fas is mediated through FADD, Caspase-8 and RIP and is inhibited by FLIP. J Cell Biol., 166:369-80.
  • Fischer, S.F., Vier, J., Kirschnek, S., Klos, A., Hess, S., Ying, S. and Häcker, G. (2004) Chlamydia inhibit host cell apoptosis by degradation of pro-apoptotic BH3-only proteins. J Exp Med., 200:905-16.
  • Chaneva, S., Schneider, G., Siegmund, D., Wajant, H., Mages, J. and Häcker, G. (2004) Enhanced Basal AP-1-Activity and Deregulation of Numerous Genes in T Cells Transgenic for a Dominant Interfering Mutant of FADD/MORT1. Eur J Immunol., 34:3006-15.
  • Fischer, S.F., Rehm, M., Bauer, A., Höfling, F., Kirschnek, S., Rutz, M., Bauer, S., Wagner, H. and Häcker, G. (2005) Toll-like receptor 9-signaling can sensitise fibroblasts for apoptosis. Immunol. Letters, 97:115-122.
  • Kirschnek, S., Ying, S., Fischer, S.F., Häcker, H., Villunger, A., Hochrein, H. and Häcker, G. (2005) Phagocytosis-induced apoptosis in macrophages is mediated by up-regulation and activation of the BH3-only protein Bim. J Immunol., 174:671-679.
  • Ying, S., Seiffert, B.M., Häcker, G. and Fischer, S.F. Broad Degradation of Pro-Apoptotic BH3-only Proteins During Infection with Chlamydia trachomatis. Infect. Immun.,73:1399-1403.
  • Fischer, S.F., Ludwig, H., Holzapfel, J., Chen, L., Huang, D.C., Sutter, G. and Häcker, G. (2006) The early proteins E3L and F1L determine the molecular balance of apoptosis inhibition and induction by Modified Vaccinia virus Ankara. Cell Death and Differentiation, 13:109-118.
  • Fischer, S. F., Vier, J., Müller-Thomas, C. and Häcker, G. (2006) Molecular events in the induction of apoptosis by Legionella pneumophila in human cells. Microbe Infect, 8:662-669.
  • Häcker. H., Redecke, V., Blagoev, B., Kratchmarova, I., Wang, G., Kamps, M.P., Saha, S.K., Oganesyan, G., Raz, E., Wagner, H., Häcker, G., Mann, M., Cheng, G. and Karin, M. (2006) Specificity in TLR Signaling: Activation of the Interferon Response Depends on TRAF3. Nature, 439:204-207.
  • Verbeke, P., Stahl, L., Ying, S., Hansen, J., Häcker, G., Darville, T. and Ojcius, D. M. (2006) AKT Phosphorylation and Recruitment of BAD by the Chlamydia Vacuole Correlate With Infection-Induced Resistance to Apoptosis. PLoS Pathogens, e45.
  • Keller, P., Schaumburg, F., Fischer, S., Häcker, G., Groß, U., and Lüder, C.G. (2006) Direct inhibition of cytochrome c-induced caspase activation in vitro by Toxoplasma gondii reveals novel mechanisms of interference with host cell apoptosis. FEMS Microbiol Letters, 258(2):312-319.
  • You, H., Pellegrini, M., Tsuchihara, Y., Yamamoto, K., Häcker, G., Erlacher, M., Villunger, A. and Mak, T. W. (2006) FOXO3a-dependent regulation of Puma in response to cytokine/growth factor withdrawal. J Exp Med, 203(7):1657-1663.
  • Bauer, A., Villunger, A., Labi, V., Fischer, S.F., Erlacher, M., Strasser, A., Wagner, H., Schmid, R.M. and Häcker, G. (2006) The NF-B regulator Bcl-3 and the BH3-only proteins Bim and Puma control the death of activated T cells. Proc Natl Acad Sci USA, 103:10979-84.
  • Ying, S., Fischer, S. F., Conte, D., Paschen, S. A., Ojcius, D. M. and Häcker, G. (2006) Characterization of Host Cell Death Induced by Chlamydia trachomatis. Infect Immun, 74:6057-66
  • Häcker, G., Suttner, K., Harada, H. and Kirschnek, S. (2006) TLR-dependent Bim phosphorylation in macrophages is mediated by ERK and is connected to proteasomal degradation of the protein. Int Immunol, 18:1749-57.
  • Erlacher, M., Labi, V., Manzl, C., Böck, G., Tzankov, A., Häcker, G., Michalak, E., Strasser, A. and Villunger, A. (2006) Bim is the rate-limiting BH3-only protein mediating cell death during lymphocyte development but cooperates with PUMA in cytokine-deprivation and stress-induced apoptosis. J Exp Med, 203:2939-51.
  • Zantl, N., Weirich, G., Hilpert, C., Seiffert, B., Fischer, S.F., Gillissen, B., Daniel, P. T. and Häcker, G. Low expression of the BH3-only protein Bim is a determinant of apoptosis resistance in renal cell carcinoma. Oncogene, May 2007 (Epub ahead of print).
  • Weber, A., Paschen, S. A., Heger, K., Wilfling, F., Frankenberg, T., Bauerschmitt, H., Seiffert, B. M., Kirschnek, S., Wagner, H. and Häcker, G. BimS Induced Apoptosis Requires Mitochondrial Localization but not Interaction with Anti-Apoptotic Bcl-2 Proteins. J Cell Biol. 177(4):625-36 (2007).
  • Bauer, A., Kirschnek, S. and Häcker, G. (2007) Inhibition of apoptosis can be accompanied by increased Bim levels in T lymphocytes and neutrophil granulocytes (Letter) Cell Death and Differentiation, 14(9):1714-6.
  • Ying, S. and Häcker, G. (2007) Apoptosis induced by direct triggering of mitochondrial apoptosis proceeds in the near-absence of some apoptotic markers. Apoptosis, 12(11):2003-11.
  • Fuchs, M., Hermannstädter, C., Haller, F., Häcker, G., Hutzler, P., Höfler, H. and Luber, B. (2008) Caspase-mediated cleavage of tumor-derived mutant E-cadherin during cisplatin-induced apoptosis. Exp Cell Res, 314(1):153-63
  • Ying, S., Christian, J. G., Paschen, S. A. and Häcker, G. (2008) Chlamydia trachomatis can protect host cells against apoptosis in the absence of cellular Inhibitor of Apoptosis Proteins and Mcl-1. Microbes and Infection, 10(1):97-101.
  • Frankenberg, T., Kirschnek, S. and Häcker, G. (2008) Phagocytosis-induced apoptosis of macrophages is linked to uptake, killing and degradation of bacteria. European Journal of Immunology, 38(1):204-15.
  • Rangelova, S., Kirschnek, S., Strasser, A. and Häcker, G. (2008) FADD and the NF-B Member Bcl-3 Regulate Complementary Pathways to T Cell Survival and Proliferation. Immunology 125(4):549-57.
  • Paschen, S., Christian, J. G., Vier, J., Schmidt, F., Walch, A., Ojcius, D. M. and Häcker. G. (2008) Cytopathicity of Chlamydia infection can be largely reproduced by expressing a single chlamydial gene, Chlamydial Protease-like Activity Factor. J Cell Biol, 182(1):117-27
  • Ying S, Pettengill M, Latham ER, Walch A, Ojcius DM, Häcker G. (2008) Premature apoptosis of Chlamydia-infected cells disrupts chlamydial development. J Infect Dis; 198(10):1536-44
  • Poeck H, Besch R, Maihoefer C, Renn M, Tormo D, Morskaya SS, Kirschnek S, Gaffal E, Landsberg J, Hellmuth J, Schmidt A, Anz D, Bscheider M, Schwerd T, Berking C, Bourquin C, Kalinke U, Kremmer E, Kato H, Akira S, Meyers R, Häcker G, Neuenhahn M, Busch D, Ruland J, Rothenfusser S, Prinz M, Hornung V, Endres S, Tüting T, Hartmann G. (2008) 5’-triphosphate-siRNA: turning gene silencing and Rig-1 activation against melanoma. Nat Med;14(11):1256-63
  • Weber, A., Kirejczyk, Z., Potthoff, S., Ploner, C. and Häcker, G. (2009) Endogenous Noxa determines the strong pro-apoptotic synergism of the BH3-mimetic ABT-737 with chemotherapeutic agents in human melanoma cells. Transl. Oncology, 2(2):73-83.
  • Lohmann, C., Muschweckh, A., Kirschnek, S., Jennen, L., Wagner, H. and Häcker, G.(2009) Induction of tumor cell apoptosis or necrosis by conditional expression of cell death proteins: analysis of cell death pathways and in vitro immune stimulatory potential. J Immunol., 182(8):4538-46.
  • Koedel, U., Frankenberg, T., Kirschnek, S., Obermaier, B., Häcker, H., Paul., R. and Häcker, G. (2009) Apoptosis is essential for neutrophil functional shutdown and determines tissue damage in experimental pneumococcal meningitis. PLoS Pathogens, May;5(5):e1000461.
  • Brenk, M, Scheler, M., Koch, S., Neumann, J., Takikawa, O., Häcker, G., Bieber, T and von Bubnoff, D. (2009) Tryptophan deprivation induces inhibitory receptors ILT3 and ILT4 on dendritic cells favoring the induction of human CD4+CD25+Foxp3+ T regulatory cells J Immunol., 183(1):145-54
  • Besch, R., Poeck, H., Hohenauer, T., Senft, D, Häcker, G., Berking, C., Hornung, V., Endres, S., Ruzicka, T., Rothenfusser, S and Hartmann, G.Proapoptotic signalling by RIG-I and MDA-5 results in tumor-specific apoptosis independent of type I interferons in melanoma. J Clin Invest.,119(8):2399-411
  • Abdul-Sater AA, Koo E, Häcker G., Ojcius DM. (2009) Inflammasome-dependent caspase-1 activation in cervical epithelial cells stimulates growth of the intracellular pathogen Chlamydia trachomatis. J Biol Chem., 284:26789-96.
  • Hippe D, Weber A, Zhou L, Chang DC, Häcker G., Lüder CG. (2009) Toxoplasma gondii infection confers resistance against BimS-induced apoptosis by preventing the activation and mitochondrial targeting of pro-apoptotic Bax. J Cell Sci., 122:3511-21
  • Weber, A., Kirejczyk, Z., Besch, R., Potthoff, S., Leverkus, M. and Häcker, G. (2010) Pro-apoptotic signalling through Toll-like receptor 3 involves TRIF-dependent activation of caspase-8 and is under the control of inhibitor of apoptosis proteins in melanoma cells. Cell Death Diff, 17(6):942-51
  • Gruber A, Cannarile MA, Cheminay C, Ried C, Marconi P, Häcker G, Brocker T. (2010) Parenchymal cells critically curtail cytotoxic T cell responses by inducing Bim-mediated apoptosis. Eur J Immunol. 40(4):966-75
  • Haider, S., Wagner, M., Schmid, M. C., Sixt, B. S., Christian, J. G., Häcker, G., Pichler, P., Müller, A., Baranyi, C., Toenshoff, E. R., Montanaro, J., Horn, M. (2010) Raman microspectroscopy reveals long-term extracellular activity of chlamydiae. Mol Microbiol, 77(3):687-700
  • Wöhrl, B., Klein, M., Rupprecht, T. A, Schmetzer, H., Angele, B., Häcker, H., Häcker, G., Pfister, H. W., Koedel, U. (2010) CXCL16 contributes to neutrophil recruitment to the CSF in pneumococcal meningitis. J Infect Dis 202(9):1389-96
  • Zall, H., Weber, A., Besch, R., Zantl, N. and Häcker, G. (2010) Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1. Molecular Cancer; 9(1):164
  • Grespi, F., Soratroi, C., Krumschnabel, G., Sohm, B, Ploner, C., Geley, S., Hengst, L., Häcker, G and Villunger, A. (2010) BH3-only protein Bmf mediates apoptosis upon inhibition of CAP-dependent protein synthesis. Cell Death Diff, 17(11):1672-83
  • von Bubnoff, D, Scheler, M., Wilms, H., Wenzel, J., von Bubnoff, N., Häcker, G., Schultze, J., Popov, A., Racz, P., Bieber, T., and Wickenhauser, C. (2011) Indoleamine 2,3-dioxygenase (IDO)-expressing myeloid dendritic cells and macrophages in infectious and non-infectious cutaneous granulomas J Am Acad Dermatol, 65(4):819-32
  • Christian, J.G., Vier, J., Paschen, S.A. and Häcker G. (2010) Cleavage of the NF-kB-family protein p65/RelA by the chlamydial protease chlamydial protease-like activity factor (CPAF) impairs pro-inflammatory signalling in cells Infected with chlamydiae. J Biol Chem., 285(53):41320-7
  • Eitz Ferrer, P., Potthoff, S., Kirschnek, S., Gasteiger, G., Kastenmüller, W., Ludwig, H., Paschen, S., Villunger, A., Sutter, G., Drexler, I. and Häcker, G. (2011) Induction of Noxa-mediated apoptosis by Modified Vaccinia Virus Ankara depends on viral recognition by cytosolic helicases, leading to IRF-3/IFN-β-dependent induction of pro-apoptotic Noxa. PLoS Path., 7(6):e1002083
  • Kirschnek, S., Vier, J., Gautam, S., Frankenberg, T., Rangelova, S., Eitz-Ferrer, P., Grespi, F., Ottina, E., Villunger, A., Häcker, H. and Häcker, G. (2011) Molecular analysis of neutrophil spontaneous apoptosis reveals a strong role for the pro-apoptotic BH3-only protein Noxa. Cell Death Diff., 18(11):1805-14
  • Feoktistova, S., Geserick, P., Kellert, B., Panayotova Dimitrova, D., Langlais, C., Hupe, M., Cain, K., MacFarlane, M., Häcker, G. and Leverkus, M. (2011) cIAPs block Ripoptosome formation, a RIP1/caspase 8 containing intracellular cell death complex differentially regulated by cFLIP isoforms. Mol. Cell, 43(3):449-63
  • Christian, J. G., Heymann, J., Paschen, S. A., Vier, J., Schauenburg, L., Rupp, J., Meyer, T. F., Häcker, G.*, ** and Heuer, D.* (2011) Targeting of a chlamydial protease impedes intracellular bacterial growth. PLoS Pathogens, 7(9):e1002283.. * Equal contribution. **, Corresponding author.
  • Wilfling, F., Weber, A., Potthoff, S., Vögtle, F.N., Meisinger, C., Paschen, S. A.* and Häcker, G.* (2012) BH3-only proteins are tail-anchored in the outer mitochondrial membrane and can initiate the activation of Bax. Cell Death Diff., 19(8):1328-36. * Equal contribution
  • Vince, J. E., Wong, W. W. L., Gentle, I., Lawlor, K. E., Allam, R, O’Reilly, L., Mason, K., Gross, O., Guarda, G., Castillo, R., Häcker, G., Silke, J. and Tschopp, J. (2012) Inhibitor of apoptosis (IAP) proteins limit IL-1 activation Immunity, 6(2):215-27
  • Pettengill, M., Marques-da-Silva, C., Avila, M., d'Arc dos Santos Oliveira, S., Lam, V., Ollawa, I., Abdul Sater, A., Coutinho-Silva, R., Häcker, G. and Ojcius, D. (2012) Reversible Inhibition of Chlamydia trachomatis Infection in Epithelial Cells Due to Stimulation of P2X4 Receptors. Infect Immun, 80(12):4232-8
  • Häcker, G. (2004). Fas – activation, development and homeostasis of T cells. In Fas Signaling (edited by Harald Wajant), Eurekah Press.
  • Häcker, G., Kirschnek, S. and Fischer, S.F. (2006) Apoptosis in infectious disease: how bacteria interfere with the apoptotic apparatus. Medical Microbiology and Immunology 195:11-19.
  • Häcker, G., Bauer, A. and Villunger, A. (2006) Apoptosis in Activated T Cells – What Are the Triggers, and What the Signal Transducers? Cell Cycle, 5:2421-4.
  • Ying, S., Pettengill, M., Ojcius, D. M. and Häcker, G. (2007) Host-Cell Survival and Death During Chlamydia Infection. Current Immunology Reviews, 3(1):31-40.
  • Häcker, G. and Weber, A. (2007) BH3-only Proteins Trigger Cytochrome c-Release, But How? Arch Biochem Biophys, 462(2):150-5.
  • Häcker, G. and Paschen, S. A. (2007) Therapeutic targets in the mitochondrial pathway. Expert Opinion in Drug Development, 11(4):515-26.
  • Paschen, S., Weber, A. and Häcker, G (2007) Mitochondrial protein import: a matter of death? Cell Cycle, 6(20):2434-9.
  • Häcker, G. Microbial inhibitors of apoptosis. In Encyclopaedia of Life Sciences.John Wiley a Sons, Ltd: Chichester
  • Häcker G. (2009) Introduction: the various deaths a cell can die, and their use in microbial infections. Microbes Infect., 11(13):1047-9.
  • Häcker G, Borner C. (2011) Preface to Mitochondria: The deadly organelle. Biochim Biophys Acta.,1813:507

Forschungsprojekte

SIGNALLING PATHWAYS – CELL DEATH AND OTHER WAYS OF RESPONDING TO SIGNALS

Life of a multi-cellular organism is determined by interactions between its cells and interactions of these cells with outside factors. In this staggering complexity we focus on a few aspects: Cell death: we are particularly interested in apoptosis as one response the cell has to respond to changes in the microenvironment. We know a lot about apoptosis but there is also a lot we don’t know, which is obviously what we are trying to learn. We use a number of cellular systems and molecular situations to understand how apoptosis is initiated and implemented in mammalian cells. A more detailed description of what we are doing at the moment is given here

Neutrophil granulocytes (neutrophils) are the first professional line of immune reaction and defence in most forms of infection (especially but not exclusively in infections with pyogenic bacteria): invading microorganisms are typically sensed by non-professional immune cells (for instance keratinocytes in the skin) and by tissue resident myeloid cells (such as macrophages). This detection causes them to secrete chemoattractants that cause neutrophils to go from the blood into the infected tissues. In this part of our work we focus mainly on neutrophils (with some attention given also to other immune cells). Neutrophils are relatively short-lived outside tissues and not easy to work with. We use mostly a system where we differentiate mouse neutrophils from progenitor cells and are studying signals determining neutrophil fate as well as signal implementation in the cells. We address questions of survival, differentiation and neutrophil contribution to immune reactions. More is given here.

Chlamydial infections, especially genital infections with Chlamydia trachomatis are a problem whose huge dimensions have only recently started to become apparent. In probably any population of young, sexually active individuals (that is people who do not engage in particularly risky behaviour) at any time there are about 5 % (one in 20) actively infected with C. trachomatis. The acute infection is typically very mild and often (especially in women) not even noticed. The problem is that the infection can stay on for long times and, in women, ascend into the pelvic regions. One of the worst possibilities is unfortunately not uncommon, namely that the prolonged infection causes infertility: the woman cannot conceive children the natural way. Chlamydia has a fascinatingly sophisticated way of growing inside human cells. We are particularly interested in a number of biological aspects of the infection: disturbance of apoptosis by Chlamydia, chlamydial proteolysis in the host cell (which we believe is required for nutrient acquisition by the bacteria), and Chlamydia-induced inflammation. Here you will find more details.

Institut für Medizinische Mikrobiologie und Hygiene

Hermann-Herder-Str. 11
D-79104 Freiburg

Ärztlicher Direktor
Prof. Dr. Georg Häcker
E-mail: georg.haecker@uniklinik-freiburg.de

Sekretariat
Kerstin Jost
Telefon: 0761 203-6532
Telefax: 0761 203-6651
E-mail: kerstin.jost@uniklinik-freiburg.de

Pforte
Bärbel Barton
Telefon: 0761 203-6510
Telefax: 0761 203-6562

Bestellung von Transportmedien
für H. pylori Resistenzbestimmungen

Telefon: 0761 203-6516
Telefax: 0761 203-6651

Lageplan