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Klinik für Innere Medizin IHämatologie, Onkologie und Stammzelltransplantation

Links of Interest

Quantitative Real-Time PCR

 

References

MIQE Guidelines - Where to start when first planning your QPCR experiment (Minimum Information for Publication of Quantitative Real-Time PCR Experiments).

Gene Quantification Home Page - this site has developed into a very useful QPCR reference web site. It summarizes many of the technical aspects of real-time PCR and has numerous software and reference downloads useful for newcomers and experts alike.

QPCR Listserver  - and its archives. This is a good place to look when trying something new.

geNorm is a very useful collection of VBA macros for Microsoft Excel which can help you determine the most stable reference genes for your experiments as well as help with normalization. 

The Ensembl Genome Browser is the home to various genome databases and is a very good reference when searching for splice site locations, etc.

Digital PCR

Gene Pi digital PCR learning center is an informative website to learn more about digital PCR, including useful statistical tools, such as how to determine the limit of blank (LOB) and limit of detection (LOD) of your assay, multiple tutorials and other information.

Digital PCR information page from the Gene Quantification Homepage.

YouTube video explaining droplet digital PCR.

Primer Design

Beacon Designer and its online tutorial - Lighthouse has a license for this program available for use in the main lab to help you with primer/probe design.

In addition to Beacon Designer, there are several possibilities for online design of primer/probe sets for QPCR. Examples of these include:

Primer 3 - online primer and probe design site from MIT's Whitehead Institute.

The RealTimeDesign software at Biosearch Technologies.

PrimerBank  - another public databank for PCR primers designed for gene expression quantification.

Company Sites

LightCycler 480 from Roche Applied Science. Also of interest may be the references and application notes for download. There are examples for relative and absolute quantification, as well as for high resolution melting analysis.

Stilla Technologies website for the Naica system

QX100 Droplet Digital PCR system from Bio-Rad.

Applied Biosystem's Sequence Detection Systems where there are a number of documents for download with general information on quantitation, as well as an ever-changing list of pre-developed TaqMan assay reagents (PDARs).

 

Universitätsklinikum Freiburg
Zentrum für Translationale Zellforschung (ZTZ)
Department of Medicine I
Tumorzentrum Freiburg - CCCF/DKTK
Center for Chronic Immune Deficiency - CCI

Contact | Information

 

FACS-Sorting Booking:

Email:
facslab@uniklinik-freiburg.de

 

Online Reservations (for machines others than cell sorters):

Booking Calender (intranet only)
MoFlo Calender (read only)

 

Head of Facility:

Marie Follo, Ph.D.
Telephone: 0761 270-77697
Telefax: 0761 270-63780
marie.follo@uniklinik-freiburg.de

 

Address:

Universitätsklinikum Freiburg
Lighthouse Core Facility

Zentrum für Translationale Zellforschung (ZTZ)
Breisacher Straße 115
D-79106 Freiburg

www.core-facility.de