Flow Cytometry
FACS AnalysisBooking of Flow Cytometers
- Please be sure that you have attended the appropriate introductory course.
- Please write your name, phone number and your laboratory name in the calendar reservation.
- Please reserve your appointments at least two days before use.
- The maximum length of a single appointment is two hours between the hours of 11:00 and 16:00. Longer appointments should be scheduled outside of this time period.
- The appointments are binding.
- If you have to cancel an appointment, please delete it from the calendar.
- Please inform the next user when you are delayed.
Overview of Lighthouse FACS Analysers
For FACS analysis we have a number of machines with different lasers and possible color combinations.
Overview of Lighthouse FACS Analysers
| 355nm | 405nm | 488nm | 561nm | 638/640nm | |
|---|---|---|---|---|---|
| Beckman Coulter Gallios / Navios 1 | - | x (2) | x (5) | - | x (3) |
| Sony Spectral Analyzer | - | (x 3) | (x 3) | - | (x 3) |
| BD FACS Canto | - | x (2) | x (4) | - | x (2) |
| BD Fortessa - Andy | - | x (2) | x (4) | - | x (2) |
| BD Fortessa - Frida | - | x (6) | x (5) | - | x (3) |
| BD Fortessa - Vincent 2 | - | x (6) | x (2) | x (5) | x (3) |
| BD Fortessa - Leonardo | - | x (7) | x (2) | x (5) | x (3) |
| BD Fortessa - Dali | x (2) | x (6) | x (5) | - | x (3) |
() - Number of possible fluorescent detectors/Laser
1 - with carousel
2 - with HTS
3 - The Sony SP6800 ZE spectral analyzer has a novel spectral detector, allowing the unmixing (separation) and analysis of multiple colors simultaneously.
Please contact the Lighthouse staff for advice on which machine would be best suited for your experiments, or for help with panel design, as well as to ask about the date and time of the next training session. For additional help with panel design, the configurations of Lighthouse analysers have been listed on the Fluorofinder website. To access them follow the links below:
See Configurations at "FluoroFinder"
Lasers & Channels of Our Flow Cytometers For Download
Fortessa Andy Lasers and Channels
Overview about lasers and suitable fluorochromes of FACS analyser ANDY.
Fortessa Frida Lasers and Channels
Overview about lasers and suitable fluorochromes of FACS analyser FRIDA.
Fortessa Leonardo Lasers and Channels
Overview about lasers and suitable fluorochromes of FACS analyser LEONARDO.
Fortessa Vincent Lasers and Channels
Overview about lasers and suitable fluorochromes of FACS analyser VINCENT.
Fortessa Dali Lasers and Channels
Overview about lasers and suitable fluorochromes of FACS analyser DALI.
BC Gallios/Navios Lasers and Channels
Overview about lasers and suitable fluorochromes of FACS analysers GALLIOS / NAVIOS.
BD FACSCanto Lasers and Channels
Overview about lasers and suitable fluorochromes of FACS analyser CANTO.
If you need more information as to which combinations of dyes or fluorescent proteins it is possible to use, please contact us. The best time to do this is when first planning a new experiment.
Offline Analysis
Lighthouse also has offline analysis workstations with several flow cytometry analysis software programs that you are welcome to use:
- FlowJo
- FACSDiva
- Kaluza
- Summit
- ModFit
- FACSArray
Cell Sorting
For information about cell sorting please see our Cell Sorting Page
Further Information
Universitätsklinikum Freiburg
Zentrum für Translationale Zellforschung (ZTZ)
Department of Medicine I
Tumorzentrum Freiburg - CCCF/DKTK
Center for Chronic Immune Deficiency - CCI
FACS-Sorting Booking:
Email:
Online Booking Calendar (new!)
Facslab-email
Online Reservations (for machines others than cell sorters until Aug. 31st. 2020):
Booking Calendar (intranet only)
Head of Facility:
Marie Follo, Ph.D.
Telephone: 0761 270-77697
Telefax: 0761 270-63780
Email: Marie Follo
Address:
Universitätsklinikum Freiburg
Lighthouse Core Facility
Zentrum für Translationale Zellforschung (ZTZ)
Breisacher Straße 115
D-79106 Freiburg