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Klinik für Innere Medizin IHämatologie, Onkologie und Stammzelltransplantation

Flow Cytometry

FACS Analysis

Booking of Flow Cytometers

  • Please write your name, phone number and your laboratory name in the calendar reservation.
  • Please reserve your appointments at least two days before use.
  • The maximum length of a single appointment is two hours between the hours of 11:00 and 16:00. Longer appointments should be scheduled outside of this time period.
  • The appointments are binding.
  • If you have to cancel an appointment, please delete it from the calendar.
  • Please inform the next user when you are delayed.

Overview of Lighthouse FACS Analysers

For FACS analysis we have a number of machines with different lasers and possible color combinations.

 

 

 

Overview of Lighthouse FACS Analysers

355nm405nm488nm561nm638/640nm
Beckman Coulter Gallios / Navios 1-x (2)x (5)-x (3)
Sony Spectral Analyzer-(x 3)(x 3)-(x 3)
BD FACS Canto-x (2)x (4)-x (2)
BD Fortessa - Frida-x (6)x (5)-x (3)
BD Fortessa - Vincent 2-x (6)x (2)x (5)x (3)
BD Fortessa - Leonardo-x (7)x (2)x (5)x (3)
BD Fortessa - Dalix (2)x (6)x (5)-x (3)

() - Number of possible fluorescent detectors/Laser 

1 - with carousel 

2 - with HTS

3 - The Sony SP6800 ZE spectral analyzer has a novel spectral detector, allowing the unmixing (separation) and analysis of multiple colors simultaneously.

Please contact the Lighthouse staff for advice on which machine would be best suited for your experiments, or for help with panel design, as well as to ask about the date and time of the next training session. For additional help with panel design, the configurations of Lighthouse analysers have been listed on the Fluorofinder website. To access them follow the links below:

See Configurations at "FluoroFinder"

Lasers & Channels of Our Flow Cytometers For Download

Fortessa Frida Lasers and Channels

Overview about lasers and suitable fluorochromes of FACS analyser FRIDA.

Download

Fortessa Leonardo Lasers and Channels

Overview about lasers and suitable fluorochromes of FACS analyser LEONARDO.

Download

Fortessa Vincent Lasers and Channels

Overview about lasers and suitable fluorochromes of FACS analyser VINCENT.

Download

Fortessa Dali Lasers and Channels

Overview about lasers and suitable fluorochromes of FACS analyser DALI.

Download

BC Gallios/Navios Lasers and Channels

Overview about lasers and suitable fluorochromes of FACS analysers GALLIOS / NAVIOS.

Download

BD FACSCanto Lasers and Channels

Overview about lasers and suitable fluorochromes of FACS analyser CANTO.

Download

Sony SP6800 Quick Start Guide

Quick Start Guide of FACS analyser Sony SP6800.

Download

If you need more information as to which combinations of dyes or fluorescent proteins it is possible to use, please contact us. The best time to do this is when first planning a new experiment.

 

Offline Analysis

Lighthouse also has offline analysis workstations with several flow cytometry analysis software programs that you are welcome to use:

  • FlowJo
  • FACSDiva
  • Kaluza
  • Summit
  • ModFit
  • FACSArray

  

Cell Sorting

For information about cell sorting please see our Cell Sorting Page

Further Information

 

Universitätsklinikum Freiburg
Zentrum für Translationale Zellforschung (ZTZ)
Department of Medicine I
Tumorzentrum Freiburg - CCCF/DKTK
Center for Chronic Immune Deficiency - CCI

Contact | Information

 

FACS-Sorting Booking:

Telephone: 
Arias: 0761 270-63770
MoFlo: 0761 270-77680

Email:
facslab@uniklinik-freiburg.de

 

Online Reservations (for machines others than cell sorters):

Booking Calender (intranet only)
MoFlo Calender (read only)

 

Head of Facility:

Marie Follo, Ph.D.
Telephone: 0761 270-77697
Telefax: 0761 270-63780
marie.follo@uniklinik-freiburg.de

 

Address:

Universitätsklinikum Freiburg
Lighthouse Core Facility

Zentrum für Translationale Zellforschung (ZTZ)
Breisacher Straße 115
D-79106 Freiburg

www.core-facility.de