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Klinik für Innere Medizin IHämatologie, Onkologie und Stammzelltransplantation


What's new at Lighthouse?

November is "Microscopy Month" at Lighthouse

During the month of November we will be hosting 3  different workshops for 3 very different types of systems/approaches to using microscopy in the life sciences which Lighthouse would like to invite you to attend.

The first workshop is on High-Content Imaging and Analysis and will take place November 12-14.

There will be a seminar discussing the approach on the morning of November 12, with chances to test and analyse your samples directly on a Perkin Elmer Operetta CLS system after that. This is a spinning disk system able to examine 3D objects or structures such as spheroids or organoids. It is also able to examine tissue sections.

MIAP HCS Workshop

Perkin Elmer Operetta CLS

November 12-14, 2019

Seminar Information:

Date/Time: Nov. 12, 2019, 9:00 - 10:30 am

Location: ZTZ, EG Seminarraum Links, 00.057

Title: The power of high-content imaging and analytics to illuminate cell population dynamics

Speaker: Dr. Achim Kirsch, Application Scientist HCS

Workshop timeslots on the Operetta are available from Nov. 12, 10:30 am until Nov. 14, 12:00 am. To reserve a slot or for more information please contact: facslab@uniklinik-freiburg

Download detailed information here  

ZEISS Workshop

Next Generation Multiplex Confocal Imaging with the New ZEISS LSM 9 Family

November 19-21, 2019

You are cordially invited to the Workshop “Next Generation Multiplex Confocal Imaging“ in which we would like to introduce you to brand new developments in live cell imaging.

Discover the ideal confocal microscope platform for 4D imaging with new ZEISS LSM 980 and ZEISS LSM 900 combined with the new Multiplex mode for parallel pixel acquisition on Airyscan 2. Convince yourself in a comprehensive workshop of lectures and system live demonstrations how your work can benefit from:

  • Multiplex mode for parallel pixel acquisition acquires up to 8 superresolution image lines with high a signal-to-noise ration (SNR) in a single sweep.
  • ZEN imaging software with Smart Setup. Sample Navigator, and Direct Processing makes your work more efficient.
  • A light efficient beam path and Airyscan 2 offer a unique combination of gentle superresolution and high SNR.

Check out the innovations in multiplex imaging directly on site in individual handson sessions.

Register now at ZEISS - limited places available

We are looking forward to welcoming you at the Workshop!


Workshop 1st Day | November 19

12.45 pm Registration
1.00 pmWelcome
1.15 pmThe New LSM 9 Family with Airyscan 2 - Adding the Multiplex Factor to Your Confocal Imaging
Andreas Lutter & Gert Sonntag, Imaging Specialists ZEISS Microscopy
2.00 pmCoffee and Discussion
2.30 pmSystem Live Demonstration: LSM 900 Airyscan 2
3.30 pmEnd


Workshop 2nd Day | November 20

Training Konfokale Mikroskopie (Deutsch)

10.00 amWillkommen
10.15 amKonfokale Mikroskopie: Grundprinzipien - der schnelle Weg zum ersten Bild
11.15 amKaffeepause
11.30 amBildaufnahme I: Aufnahmeparameter optimieren - Konfokale Detektion
12.30 pmMittagspause
1.15 pmBildaufnahme II: Mehrdimensionale Bildaufnahme: Z-Stapel, Zeitserien, Kacheln, Positionen, Fokusstrategien
2.15 pmKaffeepause
2.30 pmAiryscanning: Grundprinzipien - Airyscan FAST - Multiplexing
3.30 pmKaffeepause
3.45 pmMultichannel Unmixing - Photomanipulation - Prozessierung
4.45 pmEnde



Workshop 3rd Day | November 21

Training Confocal Microscopy (English)

10.00 amWelcome
10.15 amConfocal Microscopy: Basic Principles - The First Frames
11.15 amCoffee break
11.30 amImage Acquisition I - Optimizing imaging parameters - detectors
12.30 pmLunch
1.15 pmImage Acquisition II: Multidimensional Acquisition: z-stacks, time series, tiles, focus strategies
2.15 pmCoffee break
2.30 pmAiryscanning: Basic principles - Airyscan FAST - Multiplexing
3.30 pmCoffee break
3.45 pmMultichannel Unmixing - Photo-Manipulation - Processing
4.45 pmEnd



Universitätsklinikum Freiburg
Lighthouse Core Facility - Lecture Hall 00.057
Zentrum für Translationale Zellforschung - Klinik für Innere Medizin I
Breisacher Straße 115
79106 Freiburg

Long-Term Live Cell Imaging

The Incucyte S3 is an automated microscope located within a standard cell culture incubator and is of particular interest for low resolution, long-term, live cell imaging. It allows the independent measurement and analysis of up to 6 plates or experiments at the same time. It is the method of choice for long-term observation of spheroid and organoid development.

More information about Incucyte long-term live cell imaging...

Lighthouse Core Facility Logo

Spectral Analyzer from SONY

Our latest flow cytometer is a SONY SP6800 ZE Spectral Analyzer. It has a novel spectral detector, allowing the unmixing (separation) and analysis of multiple, even spectrally overlapping fluorochromes, simultaneously.

FACS Analyzer Introduction Appointments Now Online

Have a look at our "Trainings Page" for the upcoming events.


Universitätsklinikum Freiburg
Zentrum für Translationale Zellforschung (ZTZ)
Department of Medicine I
Tumorzentrum Freiburg - CCCF/DKTK
Center for Chronic Immune Deficiency - CCI

Contact | Information


FACS-Sorting Booking:

Arias: 0761 270-63770
MoFlo: 0761 270-77680



Online Reservations (for machines others than cell sorters):

Booking Calender (intranet only)
MoFlo Calender (read only)


Head of Facility:

Marie Follo, Ph.D.
Telephone: 0761 270-77697
Telefax: 0761 270-63780



Universitätsklinikum Freiburg
Lighthouse Core Facility

Zentrum für Translationale Zellforschung (ZTZ)
Breisacher Straße 115
D-79106 Freiburg