Klinik für Innere Medizin IHämatologie, Onkologie und Stammzelltransplantation

BD Rhapsody HT Single Cell Analysis System

The BD Rhapsody HT Single Cell Analysis System allows flexible sample processing and cell capture from hundreds to hundreds of thousands of single-cells using a gentle and robust microwell-based cartridge technology and multitier barcoding system.

Multiple samples can be processed in a single run when utilizing designated multiplexing antibodies. The captured cellular information can then be utilized to generate various types of libraries for NGS/next-generation sequencing applications.

You can easily run million-cell studies. The system is capable of capturing more than 640,000 cells per cartridge with an up to 80% cell capture rate (depending on cell type).

The system allows you to save time and sequencing costs through its visual workflow QC, allowing you to make real-time decisions before sequencing, to be certain about your cell capture for each experiment.

Process for simple single-cell capture

• Prepare single-cell suspensions
• Capture single cells
• Synthesize cDNA
• Keep cell capture beads for later processing
• NGS library prep
• Sequence cell capture beads

Available Assay Options:

RNA-Seq

• Whole transcriptome assay

• Targeted RNA-seq to focus on gene of interest with Rhapsody panel and custom option

   

TCR/BCR (VDJ)

• Full length VDJ and CDR3 assays

• Covers Gamma/Delta chains and compatible with 3’ RNA-Seq

   

AbSeq (CITE-Seq)

• Extra- and intracellular proteins
• Single pre-titrated antibodies, including custom options

• Dried down OMICS-One panels

   

 ATAC/Multiomics ATAC

• Antibody-based sample multiplexing available (lower costs/sample)

   

Sample Multiplexing (SMK)

• Human and mouse SMKs for up to 12-plex

• Species agnostic indirect labelling SMKs for up to 24-plex

   

OMICS-Guard sample preservation

• Store samples for up to 72h at 4 deg
• Antibody staining is possible before or after the preservation